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免疫检查点蛋白

免疫检查点蛋白
背景

免疫检查点(immune checkpoint)是免疫系统功能的关键调节因子。针对免疫检查点开发抗体药物用于癌症、肿瘤、自身免疫病等疾病的治疗是免疫疗法的重要手段。CTLA-4和PD-1/PD-L1是目前研究最广泛的抑制性免疫检查点途径,通过阻断免疫检查点来治疗恶性肿瘤的研究已经扩展到对LAG-3、TIM-3、TIGIT、VISTA、B7-H3等免疫共抑制分子的抗体药开发,同时针对OX40、ICOS、GITR、4-1BB、CD40等免疫共刺激分子靶向药物也处于研发阶段。因此,如何高效开展免疫检查点分子抗体药物的研发仍是目前的关注重点。

经典通路
CD155 ICOSL CD86 CD80 MHC CD40 PD-L2 PD-L1 4-1BBL OX40L GITRL MHC PD-L1 Galectin 9 TIGIT ICOS CTLA-4 CD28 TCR CD40L PD-1 PD-1 4-1BB OX40 GITR TCR PD-1 TIM-3 TCR PD-1 PD-1 LAG-3 MHC PD-L1 PD-L2 MHC-LL Dendritic cell Tumor cell Macrophage T-cells

点击分子名称查看相关产品

Stimulatory signal

Inhibitory signal

查看更多免疫检查点蛋白

产品特点&应用
产品特点:
高纯度经MALS/HPLC验证 高生物活性经ELISA/SPR/FACS等验证
可用于中和抗体验证等多种场景
严格的QC质检保证高批间一致性

主要应用场景:
产品列表

点击分子名查看详情

  • 免疫共抑制分子
  • 免疫共刺激分子
  • 其他免疫检查点分子列表
  • 抑制剂筛选试剂盒
分子 种属 标签 标记 验证数据
( 可提供 Protocol )
PD-1 Human, Mouse, Canine, Cynomolgus, Rabbit, Rat, Rhesus macaque Fc, His, Fc & His, Fc & Avi & His, Fc & Avi, His & Avi, mFc& Avi, Twin-Strep, Llama Fc , mFc Biotin-labeled, PE-labeled, Unconjugated SDS-PAGE, MALS, HPLC, ELISA, BLI, SPR,FACS
PD-L1 Human, Mouse, Rabbit, Canine, Cynomolgus / Rhesus macaque Fc, His, Fc & Avi, His & Avi, Fc & Avi & His, mFc, LlamaFc, mFc& Avi, Twin-Strep Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, BLI, SPR,FACS
PD-L2 Human, Mouse Fc, His, Fc & Avi, Avi & His, mFc Biotin-labeled, Unconjugated SDS-PAGE, HPLC, ELISA, SPR, BLI,FACS, Cell based assay
B7-H3 Human, Mouse, Cynomolgus Fc & Avi, Avi & His, His, Fc, Avi & His, , Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, Cell based assay
B7-H4 Human, Mouse His, Fc Biotin-labeled, Unconjugated SDS-PAGE, ELISA
B7-H5 Human, Mouse, Rhesus macaque Fc & Avi, Avi & His, His, Fc, mFc Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, SPR, BLI
BTLA Human, Mouse Fc & Avi, His & Avi, His, mFc, Fc Biotin-labeled, Unconjugated SDS-PAGE, ELISA
CD80(B7-1) Human, Mouse, Cynomolgus / Rhesus macaque Fc, Fc & Avi, Avi & His, mFc, His Biotin-labeled, Unconjugated SDS-PAGE, MALS, HPLC, ELISA, SPR, BLI,FACS
CD86(B7-2) Human, Mouse, Cynomolgus / Rhesus macaque Fc & Avi, Fc, His Biotin-labeled, Unconjugated SDS-PAGE, MALS, HPLC, ELISA, BLI,FACS
CD96 Human His & Avi, His, mFc Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, BLI,FACS
CD47 Human, Mouse, Rabbit, Rat, Cynomolgus / Rhesus macaque, Canine Fc, His, Fc & Avi, His & Avi, mFc, mFc & Avi, LlamaFc Biotin-labeled, FITC-labeled, Unconjugated SDS-PAGE, MALS, ELISA, SPR, BLI,FACS
CD155 Human, Mouse, Rhesus macaque Fc & Avi, His & Avi, mFc, Fc, His Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, BLI,FACS
CTLA-4 Human, Mouse, Rabbit, Rat, Cynomolgus / Rhesus macaque Fc, His, Fc & Avi, His & Avi, mFc, mFc& Avi Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, SPR, BLI,FACS
LAG-3 Human, Mouse, Rat, Cynomolgus, Marmoset Fc, His, Fc & Avi, His & Avi, mFc, mFc & Avi, LlamaFc Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, SPR, BLI,FACS
TIGIT Human, Mouse, Rabbit, Rat, Cynomolgus / Rhesus macaque, Canine mFc& Avi, Fc & Avi, His & Avi, His, Fc, mFc Biotin-labeled, Unconjugated SDS-PAGE, MALS, HPLC, ELISA, SPR, BLI
TIM-3 Human, Mouse, Cynomolgus Avi & His, mFc, Fc, His Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, SPR
CD111 Human His, Fc Unconjugated SDS-PAGE, ELISA, BLI
DNAM-1 Human, Cynomolgus Fc & Avi, His, Fc Biotin-labeled, Unconjugated SDS-PAGE, ELISA, BLI,FACS
Galectin-9 Human His, MBP & His Unconjugated SDS-PAGE
HVEM Human His, Twin-Strep, Fc, mFc, Fc & Avi Biotin-labeled, Unconjugated SDS-PAGE, ELISA, BLI
Nectin-2 Human His, Fc Biotin-labeled, Unconjugated SDS-PAGE, HPLC, ELISA, BLI
Nectin-3 Human His, Fc Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, BLI
PVRIG Human, Mouse, Cynomolgus Fc & Avi, mFc& Avi, His & Avi, His, mFc Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, BLI
SIRP alpha Human, Mouse, Cynomolgus Fc, His & Avi, mFc& Avi, mFc, His Biotin-labeled, PE-labeled, Unconjugated SDS-PAGE, MALS, HPLC, ELISA, BLI,FACS
SIRP alpha V2 Human His Biotin-labeled, Unconjugated SDS-PAGE, ELISA
SIRP gamma Human Fc Unconjugated SDS-PAGE, ELISA
CD160 Human His Unconjugated ELISA
分子 种属 标签 标记 验证数据
( 可提供 Protocol )
4-1BB Ligand Human, Mouse, Rat, Cynomolgus Fc, Fc & Avi, mFc, His & Flag Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, BLI, Cell based assay
4-1BB Human, Mouse, Rat, Rabbit, Cynomolgus / Rhesus macaque, Canine His, Fc, Fc & Avi, His & Avi, mFc Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, SPR
OX40 Ligand Human, Mouse, Rhesus macaque Fc, His, His & Avi, Avi & Fc, mFc Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, BLI,FACS
OX40 Human, Mouse, Rabbit, Rat, Rhesus macaque, Marmoset His, Fc, His & Avi, Fc & Avi, Avi & His, mFc Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, BLI,FACS
ICOS Ligand
(B7-H2)
Human, Mouse, Cynomolgus His, Fc Unconjugated SDS-PAGE, ELISA
ICOS Human, Mouse, Cynomolgus His, Fc, His & Avi Biotin-labeled, Unconjugated SDS-PAGE, ELISA
GITR Ligand Human, Mouse His, Fc, Avi & Fc Biotin-labeled, Unconjugated SDS-PAGE, ELISA
GITR Human, Mouse, Rat, Rhesus macaque, Canine His, Fc, His & Avi, Fc & Avi, mFc Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA
CD48 Human His, Fc, mFc Unconjugated SDS-PAGE, ELISA
HVEM Human His, Fc, Fc & Avi, mFc, TwiStrep Biotin-labeled, Unconjugated SDS-PAGE, MALS, BLI
Nectin-2 Human His, Fc, Fc & Avi, mFc Biotin-labeled, Unconjugated SDS-PAGE, MALS, HPLC, ELISA, BLI
CD40 Ligand Human, Mouse, Human / Rhesus macaque His, Fc, His & Avi, Avi & Fc, Avi & His & Flag, His & Flag, mFc Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, BLI
CD40 Human, Mouse, Rabbit, Cynomolgus, Rhesus macaque His, Fc, Avi & His, Fc & Avi, mFc Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, BLI,FACS
CD30 ligand Human His, mFc Unconjugated SDS-PAGE, ELISA, BLI,FACS
CD30 Human His, Fc, Avi & His, LlamaFc Biotin-labeled, PE-labeled, FITC-labeled, Unconjugated SDS-PAGE, MALS, ELISA, BLI,FACS
CD28 Mouse, Human / Cynomolgus / Rhesus macaque His, Fc, His & Avi, Fc & Avi, mFc Biotin-labeled, Unconjugated SDS-PAGE, HPLC, ELISA, SPR, BLI
CD27 Ligand Human, Mouse His & Avi, mFc, His, His & Avi & Flag, His & Flag, Fc Biotin-labeled, FITC-labeled, Unconjugated SDS-PAGE, MALS, HPLC, ELISA
CD27 Human, Mouse His, Fc, Fc & Avi, mFc Biotin-labeled, Unconjugated SDS-PAGE, MALS, HPLC, ELISA
B7-1 Human, Mouse, Cynomolgus / Rhesus macaque Fc, Fc & Avi, Avi & His, mFc, His Biotin-labeled, Unconjugated SDS-PAGE, MALS, HPLC, ELISA, SPR, BLI,FACS
B7-2 Human, Mouse, Cynomolgus / Rhesus macaque His, Fc, Fc & Avi Biotin-labeled, Unconjugated SDS-PAGE, MALS, HPLC, ELISA, BLI,FACS
LIGHT Human His, mFc, Fc Unconjugated SDS-PAGE, MALS, ELISA, BLI
B7-H7 Human, Cynomolgus His, Fc, His & Avi, Fc & Avi Biotin-labeled, Unconjugated SDS-PAGE, MALS, BLI
B7-H6 Human, Cynomolgus His, His & Avi, Fc & Avi Biotin-labeled, Unconjugated SDS-PAGE, ELISA, SPR
2B4 Human His, Fc, Avi & His Biotin-labeled, Unconjugated SDS-PAGE, ELISA
CD28H Human His, Fc Unconjugated SDS-PAGE, ELISA
NKp30 Human His, Fc Unconjugated SDS-PAGE, SPR
验证数据

可免费提供内部实验操作方法以供参考,进入产品详情页即可申请相关Protocol

MALS验证真实分子量和聚体状态

重组蛋白的均一性及聚集状态是否与天然状态保持一致是抗体药关注的重点,高纯度高均一性的产品是免疫以及抗体筛选的必要条件。

MALS验证的三聚体蛋白

CD27 Ligand MALS测定结果

The purity of Human CD27 Ligand, His,Flag Tag (active trimer) (MALS verified) (Cat. No. CDL-H52Da) is more than 95% in HP-SEC, and around 55-70 kDa verified by SEC-MALS.

高批间一致性

PD-1 MALS测点结果

Biotinylated Human PD-1 / PDCD1 Protein, Avitag™,His Tag (recommended for biopanning) (MALS verified)(Cat. No. PD1-H82E4

1.适用于免疫和抗体筛选

抗体结合验证

PD-1&PD-L1通路

PD-1 ELISA测点结果1

Immobilized Human PD-1, His Tag (Cat. No. PD1-H5221 ) at 2 μg/mL (100 μL/well) can bind Nivolumab with a linear range of 0.1-3 ng/mL.

Protocol

PD-1&PD-L1通路

PD-1 ELISA测点结果2

Serial dilutions of nivolumab were added into Human PD-1, Fc Tag, low endotoxin (Cat. No. PD1-H5257): Biotinylated Human PD-L1, Fc, Avitag, His Tag (Cat. No. PD1-H82F3) binding reactions. The half maximal inhibitory concentration (IC50) is 0.5381 μg/mL (Routinely tested).

Protocol

此外,我们还开发了PD1: PD-L1(Cat. No. EP-101)和CD47: SIRP alpha(Cat. No. EP-102)抑制剂筛选测定试剂盒,可以快速并且高通量地筛选抑制性抗体或小分子。

>>> 点击查看更多抑制剂筛选试剂盒

亲和力验证

LAG-3通路

LAG-3 BLI测定结果

Loaded Anti-LAG-3 MAb (Human IgG1) on AHC Biosensor, can bind Human LAG-3, His Tag (Cat. No. LA3-H5222) with an affinity constant of 7.47 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

LAG-3通路

LAG-3 SPR测定结果

Anti-LAG-3 MAb (Human IgG1) captured on CM5 chip via Anti-Human IgG Fc antibodies surface, can bind Human LAG-3, His Tag (Cat. No. LA3-H5222) with an affinity constant of 4.57 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

中和抗体验证

TIGIT通路

TIGIT ELISA测定结果

Serial dilutions of Human TIGIT Neutralizing antibody were added into Human TIGIT, Fc Tag (Cat. No. TIT-H5254): Biotinylated Human CD155, Fc,Avitag (Cat. No. CD5-H82F6 ) binding reactions. The half maximal inhibitory concentration (IC50) is 0.55834 μg/mL (Routinely tested).

Protocol

CD47&SIPR α通路(细胞水平验证)

CD47 FACS测定结果

FACS analysis shows that the binding of Human CD47 to ACHN expressing SIRP-a was inhibited by increasing concentration of neutralizing SIRP-a antibody. The concentration of Human CD47 used is 20 μg/mL. IC50=9.334 μg/mL

Protocol

2. 细胞功能验证

4-1BB通路

4-1BB Ligand三聚体细胞水平验证结果

Human 4-1BB Ligand (71-254), Fc Tag, active trimer (Cat. No. 41L-H5265) induce IL-8 secretion in HT1080 human CD137 cell line. The EC50 for this effect is 0.079 μg/mL (Routinely tested).

Protocol

3 药代动力学分析-PK分析

CTLA-4通路

CTLA-4二聚体细胞水平验证结果

Immobilized Human CTLA-4, His Tag, active dimer (Cat. No.CT4-H52H9) at 2 μg/mL, add increasing concentrations of CTLA-4 x OX40 Bispecific Antibody in 50% Human serum and then add Biotinylated Human OX40, Avitag,His Tag(Cat. No. TN4-H82E4) at 1 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 4 ng/mL (Intact assay, Routinely tested).

Protocol

产品评论
 
评论(362)
  1. 156XXXXXXX8
  2. 12人赞
  3. 采购本抗原进行单克隆抗体制备,以及抗体筛选等工作,本抗原经过SDS-PHAGE检测之后,条带单一,无杂蛋白,用于抗体筛选,筛选出的抗体与该抗原具体很好的亲和力
  4. >
  5. 2019-10-11
  1. 138XXXXXXX2
  2. 11人赞
  3. 用过很多种类Acro的重组蛋白,包括一些热门的靶点抗原,像LAG3、B7H3等,还有一些重组的细胞因子像IL-36α/β/γ等。说一下体验吧,外包装盒很酷炫的感觉喔~靶点抗原作为ELISA包被原料还是很好用的,跟抗体结合活性高,特异性也很强,唯一“不足”的地方可能就是价格有点子小贵吧。细胞因子类的用作过细胞功能学方面的检测,虽然刺激效果也不错,但是有些跟R&D的刺激效果比起来还是有一定改善空间,希望Acro能不断提升产品性能,越做越好!
  4. 2020-01-17
  1. 183XXXXXXX2
  2. 7人赞
  3. 该蛋白主要用于抗CD47单克隆抗体结合活性的检测,在实验方案中,使用该蛋白进行抗体Elisa活性方法开发以及活性方法检测,实验进展顺利,各批次试验之间平行性好,重复性好,与抗体结合活性好,后期用于方法验证实验。
  4. 2022-01-19
  1. 158XXXXXXX6
  2. 6人赞
  3. human PD-L1 的纯度经过SEC-HPLC检测,有很高的纯度,购买还产品用于公司产品的检测,各方面都很好,产品质量不错物流也棒棒哒,acro好多产品都经常在用,纯度都很好哦
  4. 2019-10-18
  1. 156XXXXXXX8
  2. 6人赞
  3. 在ELISA结合上,能够与CD80结合,能够获得较好的拟合曲线,获得较好的EC50值,且实验结果可重复
  4. 2020-10-09

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