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免疫检查点蛋白

背景

免疫检查点(immune checkpoint)是免疫系统功能的关键调节因子。针对免疫检查点开发抗体药物用于癌症、肿瘤、自身免疫病等疾病的治疗是免疫疗法的重要手段。CTLA-4和PD-1/PD-L1是目前研究最广泛的抑制性免疫检查点途径,通过阻断免疫检查点来治疗恶性肿瘤的研究已经扩展到对LAG-3、TIM-3、TIGIT、VISTA、B7-H3等免疫共抑制分子的抗体药开发,同时针对OX40、ICOS、GITR、4-1BB、CD40等免疫共刺激分子靶向药物也处于研发阶段。因此,如何高效开展免疫检查点分子抗体药物的研发仍是目前的关注重点。

经典通路
CD155 ICOSL CD86 CD80 MHC CD40 PD-L2 PD-L1 4-1BBL OX40L GITRL MHC PD-L1 Galectin 9 TIGIT ICOS CTLA-4 CD28 TCR CD40L PD-1 PD-1 4-1BB OX40 GITR TCR PD-1 TIM-3 TCR PD-1 PD-1 LAG-3 MHC PD-L1 PD-L2 MHC-LL Dendritic cell Tumor cell Macrophage T-cells

点击分子名称查看相关产品

Stimulatory signal

Inhibitory signal

查看更多免疫检查点蛋白

产品特点&应用
产品特点:
高纯度经MALS/HPLC验证 高生物活性经ELISA/SPR/FACS等验证
可用于中和抗体验证等多种场景
严格的QC质检保证高批间一致性

主要应用场景:
产品列表

点击分子名查看详情

  • 免疫共抑制分子
  • 免疫共刺激分子
  • 其他免疫检查点分子列表
  • 抑制剂筛选试剂盒
分子 种属 标签 标记 验证数据
( 可提供 Protocol )
PD-1 Human, Mouse, Canine, Cynomolgus, Rabbit, Rat, Rhesus macaque Fc, His, Fc & His, Fc & Avi & His, Fc & Avi, His & Avi, mFc& Avi, Twin-Strep, Llama Fc , mFc Biotin-labeled, PE-labeled, Unconjugated SDS-PAGE, MALS, HPLC, ELISA, BLI, SPR,FACS
PD-L1 Human, Mouse, Rabbit, Canine, Cynomolgus / Rhesus macaque Fc, His, Fc & Avi, His & Avi, Fc & Avi & His, mFc, LlamaFc, mFc& Avi, Twin-Strep Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, BLI, SPR,FACS
PD-L2 Human, Mouse Fc, His, Fc & Avi, Avi & His, mFc Biotin-labeled, Unconjugated SDS-PAGE, HPLC, ELISA, SPR, BLI,FACS, Cell based assay
B7-H3 Human, Mouse, Cynomolgus Fc & Avi, Avi & His, His, Fc, Avi & His, , Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, Cell based assay
B7-H4 Human, Mouse His, Fc Biotin-labeled, Unconjugated SDS-PAGE, ELISA
B7-H5 Human, Mouse, Rhesus macaque Fc & Avi, Avi & His, His, Fc, mFc Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, SPR, BLI
BTLA Human, Mouse Fc & Avi, His & Avi, His, mFc, Fc Biotin-labeled, Unconjugated SDS-PAGE, ELISA
CD80(B7-1) Human, Mouse, Cynomolgus / Rhesus macaque Fc, Fc & Avi, Avi & His, mFc, His Biotin-labeled, Unconjugated SDS-PAGE, MALS, HPLC, ELISA, SPR, BLI,FACS
CD86(B7-2) Human, Mouse, Cynomolgus / Rhesus macaque Fc & Avi, Fc, His Biotin-labeled, Unconjugated SDS-PAGE, MALS, HPLC, ELISA, BLI,FACS
CD96 Human His & Avi, His, mFc Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, BLI,FACS
CD47 Human, Mouse, Rabbit, Rat, Cynomolgus / Rhesus macaque, Canine Fc, His, Fc & Avi, His & Avi, mFc, mFc & Avi, LlamaFc Biotin-labeled, FITC-labeled, Unconjugated SDS-PAGE, MALS, ELISA, SPR, BLI,FACS
CD155 Human, Mouse, Rhesus macaque Fc & Avi, His & Avi, mFc, Fc, His Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, BLI,FACS
CTLA-4 Human, Mouse, Rabbit, Rat, Cynomolgus / Rhesus macaque Fc, His, Fc & Avi, His & Avi, mFc, mFc& Avi Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, SPR, BLI,FACS
LAG-3 Human, Mouse, Rat, Cynomolgus, Marmoset Fc, His, Fc & Avi, His & Avi, mFc, mFc & Avi, LlamaFc Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, SPR, BLI,FACS
TIGIT Human, Mouse, Rabbit, Rat, Cynomolgus / Rhesus macaque, Canine mFc& Avi, Fc & Avi, His & Avi, His, Fc, mFc Biotin-labeled, Unconjugated SDS-PAGE, MALS, HPLC, ELISA, SPR, BLI
TIM-3 Human, Mouse, Cynomolgus Avi & His, mFc, Fc, His Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, SPR
CD111 Human His, Fc Unconjugated SDS-PAGE, ELISA, BLI
DNAM-1 Human, Cynomolgus Fc & Avi, His, Fc Biotin-labeled, Unconjugated SDS-PAGE, ELISA, BLI,FACS
Galectin-9 Human His, MBP & His Unconjugated SDS-PAGE
HVEM Human His, Twin-Strep, Fc, mFc, Fc & Avi Biotin-labeled, Unconjugated SDS-PAGE, ELISA, BLI
Nectin-2 Human His, Fc Biotin-labeled, Unconjugated SDS-PAGE, HPLC, ELISA, BLI
Nectin-3 Human His, Fc Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, BLI
PVRIG Human, Mouse, Cynomolgus Fc & Avi, mFc& Avi, His & Avi, His, mFc Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, BLI
SIRP alpha Human, Mouse, Cynomolgus Fc, His & Avi, mFc& Avi, mFc, His Biotin-labeled, PE-labeled, Unconjugated SDS-PAGE, MALS, HPLC, ELISA, BLI,FACS
SIRP alpha V2 Human His Biotin-labeled, Unconjugated SDS-PAGE, ELISA
SIRP gamma Human Fc Unconjugated SDS-PAGE, ELISA
CD160 Human His Unconjugated ELISA
分子 种属 标签 标记 验证数据
( 可提供 Protocol )
4-1BB Ligand Human, Mouse, Rat, Cynomolgus Fc, Fc & Avi, mFc, His & Flag Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, BLI, Cell based assay
4-1BB Human, Mouse, Rat, Rabbit, Cynomolgus / Rhesus macaque, Canine His, Fc, Fc & Avi, His & Avi, mFc Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, SPR
OX40 Ligand Human, Mouse, Rhesus macaque Fc, His, His & Avi, Avi & Fc, mFc Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, BLI,FACS
OX40 Human, Mouse, Rabbit, Rat, Rhesus macaque, Marmoset His, Fc, His & Avi, Fc & Avi, Avi & His, mFc Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, BLI,FACS
ICOS Ligand
(B7-H2)
Human, Mouse, Cynomolgus His, Fc Unconjugated SDS-PAGE, ELISA
ICOS Human, Mouse, Cynomolgus His, Fc, His & Avi Biotin-labeled, Unconjugated SDS-PAGE, ELISA
GITR Ligand Human, Mouse His, Fc, Avi & Fc Biotin-labeled, Unconjugated SDS-PAGE, ELISA
GITR Human, Mouse, Rat, Rhesus macaque, Canine His, Fc, His & Avi, Fc & Avi, mFc Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA
CD48 Human His, Fc, mFc Unconjugated SDS-PAGE, ELISA
HVEM Human His, Fc, Fc & Avi, mFc, TwiStrep Biotin-labeled, Unconjugated SDS-PAGE, MALS, BLI
Nectin-2 Human His, Fc, Fc & Avi, mFc Biotin-labeled, Unconjugated SDS-PAGE, MALS, HPLC, ELISA, BLI
CD40 Ligand Human, Mouse, Human / Rhesus macaque His, Fc, His & Avi, Avi & Fc, Avi & His & Flag, His & Flag, mFc Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, BLI
CD40 Human, Mouse, Rabbit, Cynomolgus, Rhesus macaque His, Fc, Avi & His, Fc & Avi, mFc Biotin-labeled, Unconjugated SDS-PAGE, MALS, ELISA, BLI,FACS
CD30 ligand Human His, mFc Unconjugated SDS-PAGE, ELISA, BLI,FACS
CD30 Human His, Fc, Avi & His, LlamaFc Biotin-labeled, PE-labeled, FITC-labeled, Unconjugated SDS-PAGE, MALS, ELISA, BLI,FACS
CD28 Mouse, Human / Cynomolgus / Rhesus macaque His, Fc, His & Avi, Fc & Avi, mFc Biotin-labeled, Unconjugated SDS-PAGE, HPLC, ELISA, SPR, BLI
CD27 Ligand Human, Mouse His & Avi, mFc, His, His & Avi & Flag, His & Flag, Fc Biotin-labeled, FITC-labeled, Unconjugated SDS-PAGE, MALS, HPLC, ELISA
CD27 Human, Mouse His, Fc, Fc & Avi, mFc Biotin-labeled, Unconjugated SDS-PAGE, MALS, HPLC, ELISA
B7-1 Human, Mouse, Cynomolgus / Rhesus macaque Fc, Fc & Avi, Avi & His, mFc, His Biotin-labeled, Unconjugated SDS-PAGE, MALS, HPLC, ELISA, SPR, BLI,FACS
B7-2 Human, Mouse, Cynomolgus / Rhesus macaque His, Fc, Fc & Avi Biotin-labeled, Unconjugated SDS-PAGE, MALS, HPLC, ELISA, BLI,FACS
LIGHT Human His, mFc, Fc Unconjugated SDS-PAGE, MALS, ELISA, BLI
B7-H7 Human, Cynomolgus His, Fc, His & Avi, Fc & Avi Biotin-labeled, Unconjugated SDS-PAGE, MALS, BLI
B7-H6 Human, Cynomolgus His, His & Avi, Fc & Avi Biotin-labeled, Unconjugated SDS-PAGE, ELISA, SPR
2B4 Human His, Fc, Avi & His Biotin-labeled, Unconjugated SDS-PAGE, ELISA
CD28H Human His, Fc Unconjugated SDS-PAGE, ELISA
NKp30 Human His, Fc Unconjugated SDS-PAGE, SPR
货号 产品详情 检测范围
(μg/mL)
申请 Protocol
EP-101 PD-1 [Biotinylated] : PD-L1 Inhibitor Screening ELISA Assay Pair 10-0.078

Protocol

EP-102 CD47: SIRP alpha [Biotinylated] Inhibitor Screening ELISA Assay Pair 10-0.04

Protocol

验证数据

可免费提供内部实验操作方法以供参考,进入产品详情页即可申请相关Protocol

MALS验证真实分子量和聚体状态

重组蛋白的均一性及聚集状态是否与天然状态保持一致是抗体药关注的重点,高纯度高均一性的产品是免疫以及抗体筛选的必要条件。

MALS验证的三聚体蛋白

The purity of Human CD27 Ligand, His,Flag Tag (active trimer) (MALS verified) (Cat. No. CDL-H52Da) was more than 95% in HP-SEC, and around 55-70 kDa verified by SEC-MALS.

高批间一致性

Biotinylated Human PD-1 / PDCD1 Protein, Avitag™,His Tag (recommended for biopanning) (MALS verified)(Cat. No. PD1-H82E4

1.适用于免疫和抗体筛选

抗体结合验证

PD-1&PD-L1通路

Immobilized Human PD-1, His Tag (Cat. No. PD1-H5221 ) at 2 μg/mL (100 μL/well) can bind Nivolumab with a linear range of 0.1-3 ng/mL.

Protocol

PD-1&PD-L1通路

Serial dilutions of nivolumab were added into Human PD-1, Fc Tag, low endotoxin (Cat. No. PD1-H5257): Biotinylated Human PD-L1, Fc, Avitag, His Tag (Cat. No. PD1-H82F3) binding reactions. The half maximal inhibitory concentration (IC50) is 0.5381 μg/mL (Routinely tested).

Protocol

此外,我们还开发了PD1: PD-L1(Cat. No. EP-101)和CD47: SIRP alpha(Cat. No. EP-102)抑制剂筛选测定试剂盒,可以快速并且高通量地筛选抑制性抗体或小分子。

>>> 点击查看更多抑制剂筛选试剂盒

亲和力验证

LAG-3通路

Anti-LAG-3 MAb (Human IgG1) captured on CM5 chip via Anti-Human IgG Fc antibodies surface, can bind Human LAG-3, His Tag (Cat. No. LA3-H5222) with an affinity constant of 4.57 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Protocol

LAG-3通路

Loaded Anti-LAG-3 MAb (Human IgG1) on AHC Biosensor, can bind Human LAG-3, His Tag (Cat. No. LA3-H5222) with an affinity constant of 7.47 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

中和抗体验证

TIGIT通路

Serial dilutions of Human TIGIT Neutralizing antibody were added into Human TIGIT, Fc Tag (Cat. No. TIT-H5254): Biotinylated Human CD155, Fc,Avitag (Cat. No. CD5-H82F6 ) binding reactions. The half maximal inhibitory concentration (IC50) is 0.55834 μg/mL (Routinely tested).

Protocol

CD47&SIPR α通路(细胞水平验证)

FACS analysis shows that the binding of Human CD47 to ACHN expressing SIRP-a was inhibited by increasing concentration of neutralizing SIRP-a antibody. The concentration of Human CD47 used is 20 μg/mL. IC50=9.334 μg/mL

Protocol

2. 细胞功能验证

4-1BB通路

Human 4-1BB Ligand (71-254), Fc Tag, active trimer (Cat. No. 41L-H5265) induce IL-8 secretion in HT1080 human CD137 cell line. The EC50 for this effect is 0.079 μg/mL (Routinely tested).

Protocol

3 药代动力学分析-PK分析

CTLA-4通路

Immobilized Human CTLA-4, His Tag, active dimer (Cat. No.CT4-H52H9) at 2 μg/mL, add increasing concentrations of CTLA-4 x OX40 Bispecific Antibody in 50% Human serum and then add Biotinylated Human OX40, Avitag,His Tag(Cat. No. TN4-H82E4) at 1 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 4 ng/mL (Intact assay, Routinely tested).

Protocol

产品评论
 
评论(168)
  1. 152XXXXXXX6
  2. 4人赞
  3. 此蛋白亲和力特别好,而且比较稳定,在推荐的条件下进行溶解处理,使用时不同批次之间差异很小,在ELISA实验中,不管是用于包被还是标准品都有很好的重现性,后续还会继续使用,而且也会尝试其他蛋白。protocal也很详细,还有赠品,物流也快。
  4. >
  5. 2021-08-21
  1. 132XXXXXXX7
  2. 0人赞
  3. 买的这个带Fc标签的蛋白用来做动物免疫,因为免疫动物对蛋白的质量要求还挺高的,就跑了个电泳,做了个液相,质检结果看上去纯度还可以,希望能筛选到合适的抗体
  4. >
  5. 2021-09-01
  1. 176XXXXXXX3
  2. 1人赞
  3. 买了很多的CD47用来做结合活性,不同批次之间的蛋白质量很稳定,并且物流相当快,在我们急用而国内无货的情况下还帮我们加急放行了一批,相当给力,现在各个项目买蛋白,Acro已经成为了我们的首选。希望你们可以一如既往的为我们提供更多更好的产品。
  4. >
  5. 2021-08-13
  1. 185XXXXXXX3
  2. 2人赞
  3. 此蛋白亲和力特别好,而且比较稳定,在推荐的条件下进行溶解处理,使用时不同批次之间差异很小,在ELISA实验中,不管是用于包被还是标准品都有很好的重现性,后续还会继续使用,而且也会尝试其他蛋白。protocal也很详细,还有赠品,物流也快。
  4. >
  5. 2021-08-23
  1. 134XXXXXXX9
  2. 0人赞
  3. 在ACRO买的PD-L1 protein, His Tag. 用于PD-L1结合活性方法开发和方法验证,该蛋白响应很好,方法验证很好的完成,专属性,准确度,精密度,重复性,耐用性都通过可接受标准。实验窗口可以达到2.5倍以上。
  4. >
  5. 2021-11-03

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