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抗体同型对照——Anti-DNP 抗体

抗体同型对照——Anti-DNP 抗体
Anti-DNP 抗体背景介绍
抗体药已成为生物医药发展的一个重要领域。抗体药开发过程中的同型对照(Isotype Control)抗体通常是指不会与已知或者潜在靶点有特异结合的一抗,但在类别和亚型上,跟开发过程中的针对靶点开发的一抗保持一致。使用同型对照的目的是确定一抗的结合或效应是特异性的,排除非特异性的与Fc受体蛋白或与其他蛋白的相互作用;还可以作为竞争性结合Fc受体蛋白的抗体,与功能阻断抗体发挥同样的功能,这有利与对抗体药的药效进行有效合理的评估。非特异背景是一个需要控制的重要变量,设置对应的同型对照抗体来检测非特异性结合,从而减少假阳性结果的产生,可以更准确的评估药物开发过程中可能存在的影响因素。
DNP(2,4-二硝基苯酚)是一种半抗原,当连接到蛋白质或多肽时,可诱导强烈的免疫反应。DNP不存在于动物组织中,因此,Anti-DNP 抗体可作为抗体的优质同型对照,无内源性背景影响。ACROBiosystems 开发了一系列Human/Mouse种属、不同亚型的Anti-DNP单克隆抗体,经严格质控,特异性高,可重复性强,数据验证全面,可以在各种应用中用作抗体的同型对照,例如 ELISA、蛋白质印迹、免疫荧光、免疫组织化学和流式细胞术(FACS)等。
Anti-DNP 抗体产品列表
分子 货号 种属 产品描述 订购/预购
Anti-DNP抗体 DNP-M2 Human Monoclonal Anti-DNP antibody, Human IgG1 Isotype Control (carrier free, MALS verified)

订购

DNP-BM2 Biotinylated Monoclonal Anti-DNP antibody, Human IgG1 Isotype Control (carrier free, MALS verified)

订购

DNP-MB273 Monoclonal Anti-DNP antibody, Human IgG1 (N297A) Isotype Control (carrier free, MALS verified)

订购

DNP-M915 Monoclonal Anti-DNP antibody, Human IgG3 Isotype Control (carrier free)

订购

DNP-M3 Monoclonal Anti-DNP antibody, Human IgG4 Isotype Control (carrier free, MALS verified)

订购

DNP-FM2 FITC-Labeled Monoclonal Anti-DNP antibody, Human IgG1 Isotype Control (carrier free)

订购

DNP-M1 Mouse Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control (Carrier free, MALS verified)

订购

DNP-BM1 Biotinylated Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control (carrier free, MALS verified)

订购

DNP-M1A1 Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control

订购

DNP-BM190 Biotinylated Monoclonal Anti-DNP antibody, Mouse IgG1 Avitag™ Isotype Control

订购

DNP-FM1 FITC-Labeled Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control (carrier free)

订购

DNP-FM1A1 FITC-Labeled Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control

订购

DNP-PM1 PE-Labeled Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control (Site-specific conjugation)

订购

Anti-DNP 抗体验证数据

Anti-DNP 抗体的高纯度及结构均一度经SDS-PAGE及SEC-MALS验证

purity_ACA-H421b

Monoclonal Anti-DNP antibody, Human IgG1 (N297A) Isotype Control on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%. The purity of Monoclonal Anti-DNP antibody, Human IgG1 (N297A) Isotype Control (Cat. No. DNP-MB273) is more than 90% and the molecular weight of this protein is around 140-160 kDa verified by SEC-MALS.

purity_GCF-H5214

Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%. The purity of Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control (Cat. No. DNP-M1) is more than 90% and the molecular weight of this protein is around 130-160 kDa verified by SEC-MALS.

Anti-DNP 抗体的高生物活性经ELISA验证

DNP-M2-E1

Immobilized DNP-Albumin Conjugate at 10 μg/mL (100 μL/well) can bind Monoclonal Anti-DNP antibody, Human IgG1 Isotype Control (Cat. No. DNP-M2) with a linear range of 1-10 ng/mL (QC tested).

申请 Protocol

DNP-M3-E1

Immobilized DNP-Albumin Conjugate at 10 μg/mL (100 μL/well) can bind Monoclonal Anti-DNP antibody, Human IgG4 Isotype Control (Cat. No. DNP-M3) with a linear range of 1-15 ng/mL (QC tested).

申请 Protocol

DNP-MB273-E1

Immobilized DNP-Albumin Conjugate at 10 μg/mL (100 μL/well) can bind Monoclonal Anti-DNP antibody, Human IgG1 (N297A) Isotype Control (Cat. No. DNP-MB273) with a linear range of 1-10 ng/mL (QC tested).

申请 Protocol

DNP-M1-E1

Immobilized DNP-Albumin Conjugate, Bovine at 10 μg/mL (100 μL/well) can bind Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control (Cat. No. DNP-M1) with a linear range of 2-20 ng/mL (QC tested).

申请 Protocol

Anti-DNP 抗体可用作同型对照经FACS验证

DNP-BM1-F3

2e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1 μg/mL of Biotinylated Anti-DNP Antibody, Mouse IgG1 (Cat. No. DNP-BM1) and positive control respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).

申请 Protocol

DNP-FM1-F3

2e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1 μg/mL of FITC-Labeled Anti-DNP Antibody, Mouse IgG1 (Cat. No. DNP-FM1) and positive control respectively, and FITC signal was used to evaluate the binding activity (Routinely tested).

申请 Protocol

DNP-M1A1-F1

2e5 of anti-CD19 CAR-293 cells were stained with 100ul of 1:50 dilution of Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control (Cat. No. DNP-M1A1) and positive control antibody respectively, washed and then followed by PE anti-mouse IgG1 and analyzed with FACS (QC tested).

申请 Protocol

DNP-BM190-F1

2e5 of anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) Biotinylated Monoclonal Anti-DNP antibody, Mouse IgG1 Avitag Isotype Control (Cat. No. DNP-BM190) and positive control antibody (Cat. No. FM3-BY54) respectively, washed and then followed by PE-SA and analyzed with FACS (QC tested).

申请 Protocol

DNP-FM1A1-F1

2e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of FITC-Labeled Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control (Cat. No. DNP-FM1A1) and positive control (Cat. No. FM3-FY45P1) respectively, and FITC signal was used to evaluate the binding activity (QC tested).

申请 Protocol

DNP-PM1-F1

5e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control (Cat. No. DNP-PM1) and positive control (Cat. No. FM3-HPY53) respectively, and PE signal was used to evaluate the binding activity (QC tested).

申请 Protocol

其他同型对照产品

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