Recombinant Proteins iso9001 ISO13485
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Human Nectin-4 Protein, His Tag

分子别名(Synonym)

NECTIN4,LNIR, PRR4, PVRL4

表达区间及表达系统(Source)

Human Nectin-4, His Tag (NE4-H52H3) is expressed from human 293 cells (HEK293). It contains AA Gly 32 - Ser 349 (Accession # Q96NY8-1).

Predicted N-terminus: Gly 32

蛋白结构(Molecular Characterization)

Online(Gly 32 - Ser 349) Q96NY8-1

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 36.0 kDa. The protein migrates as 40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human Nectin-4, His Tag (Cat. No. NE4-H52H3) SDS-PAGE gel

Human Nectin-4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

获取HPLC数据

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human Nectin-4, His Tag (Cat. No. NE4-H52H3) at 5 μg/mL (100 μL/well) can bind Human PVRL1, Fc Tag with a linear range of 0.01-0.313 μg/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Human Nectin-4, His Tag (Cat. No. NE4-H52H3) at 1 μg/mL (100 μL/well) can bind Mouse Anti-Nectin-4 Antibody with a linear range of 0.2-2 ng/mL (QC tested).

Protocol

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Human Nectin-1, Fc Tag (Cat. No. PV1-H5253) on Protein A Biosensor, can bind Human Nectin-4, His Tag (Cat. No. NE4-H52H3) with an affinity constant of 0.271 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

Biotinylated Human BLI

Loaded Human Nectin-4, His Tag (Cat. No. NE4-H52H3) on HIS1K Biosensor, can bind Human Nectin-1, Fc Tag (Cat. No. PV1-H5253) with an affinity constant of 0.24 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

 

背景(Background)

Nectin-4 (gene name PVRL4, poliovirus receptor-like 4) is a 66 kDa type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. Nectins are cell adhesion molecules that play a key role in various biological processes such as polarity, proliferation, differentiation and migration, for epithelial, endothelial, immune and neuronal cells, during development and adult life. Nectin-4 is a tumor-associated antigen in 50%, 49% and 86%o of breast, ovarian and lung carcinomas, respectively, mostly on tumors of bad prognosis. Its expression is not detected in the corresponding normal tissues.

 

前沿进展

 
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Nectin-4靶点信息
英文全称:Nectin-4
中文全称:细胞黏附分子nectin-4
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:1详情
最高研发阶段:批准上市
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