ActiveMax®是ACROBiosystems百普赛斯基于高活性重组蛋白和生物相容性磁珠开发生产技术平台,开发的一系列细胞激活、培养等应用场景的磁珠产品品牌,如靶点特异性激活磁珠、CD3/CD28抗体偶联磁珠等,经严格质量控制,具有无动物源性、无菌、无支原体、超低内毒素等安全特性,适用于免疫细胞治疗相关研究和临床前工艺开发,且可定制GMP级别磁珠以应用于商业化生产的无缝衔接。
此外,我们还提供无菌细胞级别链酶亲和素磁珠,可帮助您快速实现各种细胞级别蛋白/抗体偶联磁珠的个性化制备。
靶点特异性磁珠
细胞激活
利用基于磁珠偶联的抗原多聚体的方式可很好的特异性激活CAR-T细胞。
CAR细胞富集
特异性靶点磁珠可用于富集低丰度CAR细胞或稀有CAR-T细胞。
CAR细胞体外分析
采用特异性靶点偶联磁珠与CAR-T 细胞共孵育后,进行如T细胞激活、增殖、细胞因子释放等体外分析来定位产品表征及放行测试。
抗体偶联磁珠
链酶亲和素偶联磁珠
ActiveMax Streptavidin coupled μbeads, premium grade coupled with protein. 40ug of the biotinylated human BCMA protein (Cat. No. BCA-H82E4) was added to 1mg of ActiveMax Streptavidin coupled μbeads, premium grade (for cells) (Cat. No. MBS-C009), mix well and incubate for 60 mins at room temperature. And then the coupled μbeads were fluorescently stained using PE labeled anti-human BCMA antibody and analyzed by flow cytometry.
ActiveMax Streptavidin coupled μbeads, premium grade coupled with human BCMA protein. The biotinylated human BCMA protein (Cat. No. BCA-H82E4) in serial dose was added to 1mg of ActiveMax Streptavidin coupled μbeads, premium grade (for cells) (Cat. No. MBS-C009), mix well and incubate for 60 mins at room temperature. And then the unbound protein in supernatant measured by Micro BCA Protein Assay Kit was subtracted to calculate the BCMA protein coupled amount on the μbeads (loaded capacity).
ActiveMax Streptavidin coupled μbeads, premium grade coupled with antibody. 50ug of the biotinylated anit-FMC63 Antibody (Cat. No. FM3-BY54) was added to 1mg of ActiveMax Streptavidin coupled μbeads, premium grade (for cells) (Cat. No. MBS-C009), mix well and incubate for 60 mins at room temperature. And then the coupled μbeads were fluorescently stained using PE labeled anti-mouse Fc antibody and analyzed by flow cytometry.
ActiveMax Streptavidin coupled μbeads, premium grade coupled with antibody. The anit-FMC63 Antibody (Cat. No. FM3-BY54) in serial dose was added to 1mg of ActiveMax Streptavidin coupled μbeads, premium grade (for cells) (Cat. No. MBS-C009), mix well and incubate for 60 mins at room temperature. And then the unbound protein in supernatant measured by Micro BCA Protein Assay Kit was subtracted to calculate the antibody coupled amount on the μbeads (loaded capacity).