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CD39/CD73:肿瘤免疫腺苷酸通路明星靶点

背景
肿瘤微环境(TME)是肿瘤免疫治疗的重要影响因素之一,CD39/CD73腺苷通路在肿瘤微环境的先天性和适应性免疫中发挥着重要作用。CD39是一种二三磷酸核苷酸水解酶1(NTPDase 1),存在于细胞表面,CD73是一种胞外-5′-核苷酸酶(NT5E),以同源二聚体形式存在于细胞表面,也可以游离形式存在于胞外。
肿瘤微环境中的ATP可有效激活免疫细胞活性;而胞外ATP、ADP在CD39催化作用下分解产生腺苷单磷酸(AMP);AMP在CD73催化作用下生成的ADO(腺苷);CD73可通过结合腺苷受体、激活蛋白激酶A(PKA)和Csk激酶,抑制LCK、MAPK、PKC等一系列与免疫激活相关的信号通路,进而导致肿瘤免疫逃逸。
为加速CD39/CD73药物研发,ACROBiosystems提供CD39和CD73高酶活系列蛋白产品,酶活稳定,纯度和活性经验证,可以免费提供活性验证Protocol,以供客户进行抗体药物免疫与筛选。
产品列表
分子名 货号 物种 产品描述
CD73CD3-C52H5CanineCanine CD73 / NT5E Protein, His Tag
CD3-C52H9CynomolgusCynomolgus CD73 Protein, His Tag (active enzyme)
CD3-H5252HumanHuman CD73 Protein, Mouse IgG2a Fc Tag (active enzyme)
CD3-H52H7HumanHuman CD73 / NT5E Protein, His Tag (HPLC-verified) (active enzyme)
CD3-H82E3HumanBiotinylated Human CD73 / NT5E Protein, His,
CD3-M52H9MouseMouse CD73 / NT5E Protein, His Tag (active enzyme)
CD3-R52H3RabbitRabbit CD73 / NT5E Protein, His Tag (active enzyme)
CD3-S52H3Sus scrofa (Pig)Sus scrofa CD73 / NT5E Protein, His Tag (active enzyme, MALS verified)
MBS-K022HumanHuman CD73-coupled Magnetic Beads
CD39CD9-C52H3CynomolgusCynomolgus CD39 / ENTPD1 Protein, His Tag
CD9-H5253HumanHuman CD39 Protein, Mouse IgG2a Fc Tag
CD9-H52H4HumanHuman CD39 / ENTPD1 Protein, His Tag (active enzyme)
CD9-H82E5HumanBiotinylated Human CD39 / ENTPD1 Protein, His, (MALS verified) (active enzyme)
CD9-M52H3MouseMouse CD39 / ENTPD1 Protein, His Tag
*如果您希望ACRO开发更多类型的腺苷酸通路蛋白产品, 请 点击提议
*如果您希望ACRO补充更多CD39/CD73蛋白产品的实验设计和应用数据,请 点击咨询
产品验证数据
高酶活
测定产品 Human CD39 / ENTPD1 Protein, His Tag Human CD73 / NT5E Protein, His Tag (HPLC-verified)
水解底物 腺苷-5 ' -三磷酸(ATP) 腺苷-5 ' -单磷酸(AMP)
酶活
(pmol/min/μg)
ACRO产品(货号:CD9-H52H4 竞品  ACRO产品(货号:CD3-H52H7 竞品 
> 6,000 >5,000 >37,000 >15,000

注:同一产品在相同条件下测定酶活

高纯度经HPLC验证

The purity of Human CD73, His Tag (HPLC-verified) (Cat. No. CD3-H52H7) was greater than 90% as determined by SEC-HPLC.

Protocol

高生物活性
ELISA

Immobilized Human CD39, His Tag (Cat. No. CD9-H52H4) at 1 μg/mL (100 μL/well) can bind Anti-CD39 Antibody, Human IgG1 with a linear range of 0.1-0.8 ng/mL (QC tested).

Protocol

Immobilized 62 μg CD73 protein to 1 mg Beads can bind the Anti-CD73 Antibody with an EC50 of 0.5659 μg/mL (QC tested).

Protocol

SPR

Anti-CD73 antibody (Human IgG1) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human CD73, His Tag (HPLC-verified) (Cat. No. CD3-H52H7) with an affinity constant of 0.164 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Protocol



热门评论
ACROBiosystems提供的高质量CD39/CD73系列蛋白产品客户反馈良好。
 
评论(3)
  1. 182XXXXXXX2
  2. 1人赞
  3. Mouse CD73到货后就立即开始使用啦,虽然是鼠的,但是与抗体的结合力依然很强,BLI数据显示亲和力有6E-10.
  4. >
  5. 2020-01-10
  1. 182XXXXXXX2
  2. 2人赞
  3. Human CD73用来检测medimmune家的抗体与其亲和力,结果具有很高的亲和力,亲和力为5E-10.
  4. >
  5. 2020-01-10
  1. 136XXXXXXX6
  2. 10人赞
  3. 购买蛋白用于测试CD39封闭性抗体封闭CD39水解ATP的功能实验。通过系列梯度CD39抗体与一定浓度的CD39(0.5ug/ml)孵育,再加入50uM ATP,用CTG检测,得到很好的曲线。证明该蛋白具有很好的ATP酶水解功能。
  4. 2019-11-11

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