膜杰作
Star Staining
抗体来源（Source）
Anti-FITC Antibody, Rabbit IgG-Acridinium ester (F11) is a chimeric monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which combines the variable region of a mouse monoclonal antibody with human IgG1 constant domain. The mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with FITC.
亚型（Isotype）
Rat IgG | Kappa
特异性（Specificity）
This product is a specific antibody specifically reacts with FITC.
标记（Labeling）
Acridinium ester, can react with the primary amino group of protein. Under alkaline conditions, NHS is replaced as the leaving group, and the protein forms a stable amide bond with Acridinium ester.
蛋白标记度（Protein Ratio）
Passed as determined by binding MPCLIA.
应用（Application）
MPCLIA
纯度（Purity）
>90% as determined by SDS-PAGE.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Anti-FITC Antibody, Rabbit IgG-Acridinium ester (F11) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Anti-FITC Antibody, Rabbit IgG-Acridinium ester (F11) (Cat. No. FIC-S288) is more than 0.95 and the molecular weight of this protein is around 135-160 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-MPCLIA
Immobilized 0.05 μg /Test of FITC-Labeled Human BCMA, His Tag (Cat. No. BCA-HF2H1) to the Anti-FITC Antibody, Rabbit IgG-Acridinium ester (F11) (MALS verified) (Cat. No. FIC-S288, 0.04 μg /Test), incubated with 100 μL /Test of Monoclonal Anti-Human BCMA Antibody, Human IgG1 (Cat. No. BCA-M26) at increasing concentration coupled to Anti-Human IgG-coupled Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A004) (10 μg beads/Test). Detection was performed with sensitivity of 3.9 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).
Protocol
The MPCLIA assay shows that Anti-FITC Antibody, Rabbit IgG-Acridinium ester (F11) (MALS verified) (Cat. No. FIC-S288) is stable at 37°C for 48 hours.
The MPCLIA assay shows that Anti-FITC Antibody, Rabbit IgG-Acridinium ester (F11) (MALS verified) (Cat. No. FIC-S288) is stable after freezing and thawing 3 times.
背景（Background）
Chemiluminescence immunoassay (CLIA) is a much more effective measurement with the advantages of high sensitivity, uniformity and broad dynamic range.
However, in traditional chemiluminescent immunoassay (CLIA),microplate was applied to immobilize antigen or antibody by physical absorption, which holds a negative effect on the perfor-mance of assay because of the low specific surface area of micro-plate.
The use of micromagnetic particles (MMPs) have proven to be a support for addressing the shortage of traditional CLIA mentioned above The chemiluminescent immunoassay is a technique used for disease diagnosis, drug development, chemical reaction monitoring, and many other applications.
The MMPs-based chemiluminescence immunoassays could improve the surface area for immobilization, capture efficiency and accuracy of the assay, thus obtaining higher sensitivity and shorter analysis time.
