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>产品&服务 > Star Staining——新一代荧光定点标记技术平台及系列产品

Star Staining——新一代荧光定点标记技术平台及系列产品

Star Staining-新一代荧光定点标记技术平台及系列产品
背景
ACROBiosystems百普赛斯Star Staining新一代定点标记技术平台开发的高质量荧光标记蛋白,可保持蛋白的天然构象,不影响蛋白活性,均一性好、批间一致性高。“星级”的研发实力,“星级”的技术平台及产品,
Star Staining专为CAR-T细胞质控和临床样本分析检测而设计。
流式细胞术是细胞治疗药物研发过程中检测CAR阳性T细胞的重要技术手段,荧光标记蛋白因其预先标记了荧光素基团,可通过一步染色完成检测,既简化了检测流程、节省了检测时间,又避免了因使用二抗产生的非特异背景,成为流式检测CAR表达的首选试剂。
然而,荧光标记蛋白的选择面临着诸多难题。一方面,由于市面上主流的蛋白标记技术为传统随机化学标记方法,易改变蛋白的天然结构,导致活性下降,降低检测灵敏度而无法检测低丰度的CAR,另外,使用随机化学标记技术,易造成蛋白聚集,在进行FACS检测时会产生非特异信号,且标记工艺不稳定导致荧光蛋白产品不均一,难以控制批间差,因此,使用传统随机化学标记方法,很难获得高活性、高批间一致性的荧光标记蛋白,从而影响CAR-T药物的质量控制,更难以用于临床分析应用;另一方面,在进行多重染色时,需要选择多种荧光染料的标记蛋白,或流式检测设备所限,仅能选择用于该设备所匹配的荧光,因此,丰富的荧光类型,也是FACS检测CAR表达时所考虑的要点。
为解决上述问题,ACROBiosystems凭借Star Staining新一代定点标记技术平台,成功开发了一系列荧光标记类型的CAR靶点相关蛋白产品,以“星级”的产品优势,全方位满足CAR-T阳性率检测的不同需求。Star Staining新一代定点标记技术平台可对蛋白的特定位点进行标记,标记速度快效率高,不影响蛋白的天然空间构象,活性好检测灵敏度高,产物均一性好,无非特异信号,这种独特的定点标记技术成为开发高质量荧光标记蛋白产品的有力武器。
技术原理
传统化学标记技术
传统化学标标记技术
非选择性随机标记,影响蛋白正常结构
蛋白活性位点被覆盖
改变蛋白亲水性,蛋白易聚集
产品批间差异大
新一代定点标记技术
新一代定点标记技术
蛋白特定位点标记
标记位点远离活性中心,维持蛋白天然空间构象
高效生物正交反应,无非特异信号
产物均一性好、批间一致性高
Star Staining特色

ACROBiosystems百普赛斯致力于开发适用于细胞治疗产品质控和临床样本分析的更高质量标准检测试剂产品,在多年技术经验积累的基础上,不断更新完善,以“星级”研发实力,成功搭建了“星级标准”的Star Staining新一代荧光定点标记技术平台,开发了独具特色的Star Staining系列荧光定点标记蛋白产品,助力细胞治疗药物的研发进程。

采用新一代定点标记技术, 保持蛋白天然构象,高活性

高灵敏度和高特异性,经类CAR细胞批检验证

极低非特异信号,经PBMC双染批检放行

高度均一和批间一致性,高水平满足临床样本分析检测要求

热门产品
  • FITC
  • PE
  • APC
  • Alexa Fluor 647
  • Alexa Fluor 555
  • Alexa Fluor 488
分子 货号 产品描述 订购/预购
BCMA BCA-HF2H3 FITC-Labeled Human BCMA / TNFRSF17 Protein, His TagStar Staining

订购

CD19 CD9-HF2H3 FITC-Labeled Human CD19 (20-291) Protein, His TagStar Staining

订购

CD27 Ligand CDL-HF249 FITC-Labeled Human CD27 Ligand / CD70 Protein, His TagStar Staining

订购

GUCY2C GUC-HF2H9 FITC-Labeled Human GUCY2C / Guanylyl cyclase C Protein, His TagStar Staining

订购

Mesothelin MSN-HF2H3 FITC-Labeled Human Mesothelin / MSLN (296-580) Protein, His TagStar Staining

订购

分子 货号 产品描述 订购/预购
BCMA BCA-HP2H7 PE-Labeled Human BCMA / TNFRSF17 Protein, His TagStar Staining

订购

CD19 CD9-HP2H5 PE-Labeled Human CD19 (20-291) Protein, His TagStar Staining

订购

CD27 Ligand CDL-HP246 PE-Labeled Human CD27 Ligand / CD70 Protein, His TagStar Staining

订购

CD4 CD4-HP2H8 PE-Labeled Human CD4 Protein, His TagStar Staining

订购

CD7 CD7-HP2H6 PE-Labeled Human CD7 Protein, His TagStar Staining

订购

CLEC12A CLA-HP245 PE-Labeled Human CLEC12A / MICL / CLL-1 Protein, His TagStar Staining

订购

EGF R EGR-HP2H7 PE-Labeled Human EGF R Protein, His TagStar Staining

订购

EGFRvIII EGI-HP2H6 PE-Labeled Human EGFRvIII Protein, His TagStar Staining

订购

FOLR1 FO1-HP2H6 PE-Labeled Human FOLR1 Protein, His TagStar Staining

订购

Glypican 3 GP3-HP2H9 PE-Labeled Human Glypican 3 / GPC3 Protein, His TagStar Staining

订购

GUCY2C GUC-HP2H9 PE-Labeled Human GUCY2C / Guanylyl cyclase C Protein, His TagStar Staining

订购

Her2 HE2-HP2H8 PE-Labeled Human Her2 / ErbB2 Protein, His TagStar Staining

订购

Mesothelin MSN-HP2H8 PE-Labeled Human Mesothelin / MSLN (296-580) Protein, His TagStar Staining

订购

ROR1 RO1-HP2H5 PE-Labeled Human ROR1 Protein, His TagStar Staining

订购

Siglec-2 SI2-HP2H5 PE-Labeled Human Siglec-2 / CD22 Protein, His TagStar Staining

订购

Siglec-3 CD3-HP2H7 PE-Labeled Human Siglec-3 / CD33 Protein, His TagStar Staining

订购

SLAMF7 SL7-HP2H6 PE-Labeled Human SLAMF7 / CRACC / CD319 Protein, His TagStar Staining

订购

分子 货号 产品描述 订购/预购
BCMA BCA-HA2H4 APC-Labeled Human BCMA / TNFRSF17 Protein, His TagStar Staining

订购

CD19 CD9-HA2H9 APC-Labeled Human CD19 (20-291) Protein, His TagStar Staining

订购

CD27 Ligand CDL-HA248 APC-Labeled Human CD27 Ligand / CD70 Protein, His TagStar Staining

订购

CD4 CD4-HA2H9 APC-Labeled Human CD4 Protein, His TagStar Staining

订购

CD7 CD7-HA2H6 APC-Labeled Human CD7 Protein, His TagStar Staining

订购

CLEC12A CLA-HA244 APC-Labeled Human CLEC12A / MICL / CLL-1 Protein, His TagStar Staining

订购

DLL3 DL3-HA2H8 APC-Labeled Human DLL3 Protein, His TagStar Staining

订购

EGF R EGR-HA2H8 APC-Labeled Human EGF R Protein, His TagStar Staining

订购

EGFRvIII EGI-HA2H5 APC-Labeled Human EGFRvIII Protein, His TagStar Staining

订购

FOLR1 FO1-HA2H8 APC-Labeled Human FOLR1 Protein, His TagStar Staining

订购

Glypican 3 GP3-HA2H7 APC-Labeled Human Glypican 3 / GPC3 Protein, His TagStar Staining

订购

GUCY2C GUC-HA2H7 APC-Labeled Human GUCY2C / Guanylyl cyclase C Protein, His TagStar Staining

订购

Her2 HE2-HA2H9 APC-Labeled Human Her2 / ErbB2 Protein, His TagStar Staining

订购

Mesothelin MSN-HA2H6 APC-Labeled Human Mesothelin / MSLN (296-580) Protein, His TagStar Staining

订购

ROR1 RO1-HA2H4 APC-Labeled Human ROR1 Protein, His TagStar Staining

订购

Siglec-2 SI2-HA2H4 APC-Labeled Human Siglec-2 / CD22 Protein, His TagStar Staining

订购

Siglec-3 CD3-HA2H3 APC-Labeled Human Siglec-3 / CD33 Protein, His TagStar Staining

订购

分子 货号 产品描述 订购/预购
BCMA BCA-HA2H9 Alexa Fluor™ 647-Labeled Human BCMA / TNFRSF17 Protein, His TagStar Staining

订购

CD19 CD9-HA2H6 Alexa Fluor™ 647-Labeled Human CD19 (20-291) Protein, His TagStar Staining

订购

CD27 Ligand CDL-HA246 Alexa Fluor™ 647-Labeled Human CD27 Ligand / CD70 Protein, His TagStar Staining

订购

DLL3 DL3-HA2H3 Alexa Fluor™ 647-Labeled Human DLL3 Protein, His TagStar Staining

订购

FMC63 FM3-AM534 Alexa Fluor™ 647-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45)Star Staining

订购

GUCY2C GUC-HA2H8 Alexa Fluor™ 647-Labeled Human GUCY2C / Guanylyl cyclase C Protein, His TagStar Staining

订购

Her2 HE2-HA2H5 Alexa Fluor™ 647-Labeled Human Her2 / ErbB2 Protein, His TagStar Staining

订购

HLA-A*1101 & B2M & KRAS (VVVGAGGVGK) HLS-HA2H8 Alexa Fluor™ 647-Labeled Human HLA-A*11:01&B2M&KRAS (VVVGAGGVGK) Complex ProteinStar Staining (Monomer)

订购

HLA-A*1101 & B2M & WT1 (FSCRWPSCQK) HLW-HA2H4 Alexa Fluor™ 647-Labeled Human HLA-A*11:01&B2M&WT-1 (FSCRWPSCQK) Tetramer ProteinStar Staining

订购

HLA-A*1101 | B2M | KRAS (VVGAGGVGK) HLS-HA2H9 Alexa Fluor™ 647-Labeled Human HLA-A*11:01&B2M&KRAS (VVGAGGVGK) Complex ProteinStar Staining (Monomer)

订购

Mesothelin MSN-HA2H5 Alexa Fluor™ 647-Labeled Human Mesothelin / MSLN (296-580) Protein, His TagStar Staining

订购

Siglec-3 CD3-HA2H6 Alexa Fluor™ 647-Labeled Human Siglec-3 / CD33 Protein, His TagStar Staining

订购

分子 货号 产品描述 订购/预购
BCMA BCA-HA2H7 Alexa Fluor™ 555-Labeled Human BCMA / TNFRSF17 Protein, His TagStar Staining

订购

CD19 CD9-HA2H7 Alexa Fluor™ 555-Labeled Human CD19 (20-291) Protein, His TagStar Staining

订购

CD27 Ligand CDL-HA245 Alexa Fluor™ 555-Labeled Human CD27 Ligand / CD70 Protein, His TagStar Staining

订购

GUCY2C GUC-HA2H6 Alexa Fluor™ 555-Labeled Human GUCY2C / Guanylyl cyclase C Protein, His TagStar Staining

订购

Her2 HE2-HA2H4 Alexa Fluor™ 555-Labeled Human Her2 / ErbB2 Protein, His TagStar Staining

订购

Mesothelin MSN-HA2H8 Alexa Fluor™ 555-Labeled Human Mesothelin / MSLN (296-580) Protein, His TagStar Staining

订购

Siglec-2 SI2-HA2H9 Alexa Fluor™ 555-Labeled Human Siglec-2 / CD22 Protein, His TagStar Staining

订购

分子 货号 产品描述 订购/预购
BCMA BCA-HA2H8 Alexa Fluor™ 488-Labeled Human BCMA / TNFRSF17 Protein, His TagStar Staining

订购

CD19 CD9-HA2H3 Alexa Fluor™ 488-Labeled Human CD19 (20-291) Protein, His TagStar Staining

订购

CD27 Ligand CDL-HA247 Alexa Fluor™ 488-Labeled Human CD27 Ligand / CD70 Protein, His TagStar Staining

订购

CD7 CD7-HA2H9 Alexa Fluor™ 488-Labeled Human CD7 Protein, His TagStar Staining

订购

EGFRvIII EGI-HA2H7 Alexa Fluor™ 488-Labeled Human EGFRvIII Protein, His TagStar Staining

订购

Glypican 3 GP3-HA2H5 Alexa Fluor™ 488-Labeled Human Glypican 3 / GPC3 Protein, His TagStar Staining

订购

GUCY2C GUC-HA2H5 Alexa Fluor™ 488-Labeled Human GUCY2C / Guanylyl cyclase C Protein, His TagStar Staining

订购

Her2 HE2-HA2H3 Alexa Fluor™ 488-Labeled Human Her2 / ErbB2 Protein, His TagStar Staining

订购

HLA-A*1101 & B2M & KRASG12D (VVVGADGVGK) HLD-HA2H4 Alexa Fluor™ 488-Labeled Human HLA-A*11:01&B2M&KRASG12D (VVVGADGVGK) Complex ProteinStar Staining (Monomer)

订购

HLA-A*1101 | B2M | KRASG12D (VVGADGVGK) HLD-HA2H7 Alexa Fluor™ 488-Labeled Human HLA-A*11:01&B2M&KRASG12D (VVGADGVGK) Complex ProteinStar Staining (Monomer)

订购

Mesothelin MSN-HA2H9 Alexa Fluor™ 488-Labeled Human Mesothelin / MSLN (296-580) Protein, His TagStar Staining

订购

ROR1 RO1-HA2H7 Alexa Fluor™ 488-Labeled Human ROR1 Protein, His TagStar Staining

订购

Siglec-2 SI2-HA2H7 Alexa Fluor™ 488-Labeled Human Siglec-2 / CD22 Protein, His TagStar Staining

订购

验证数据

纯度高于90%

Star Staining FITC-labeled Human BCMA产品纯度高于90%。
BCMA SDS-PAGE

FITC-Labeled Human BCMA, His Tag (Cat. No. BCA-HF2H3) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

BCMA SEC-MALS

The purity of FITC-Labeled Human BCMA, His Tag (Cat. No. BCA-HF2H3) is more than 90% and the molecular weight of this protein is around 24-34 kDa verified by SEC-MALS.

高灵敏度

Star Staining FITC-labeled Human BCMA和CD19蛋白产品的检测灵敏度显著高于竞品。
BCMA 灵敏度FACS验证数据图
CD19 灵敏度FACS验证数据图

Binding activity of FITC-Labeled Human BCMA and CD19 protein from two different vendors were evaluated by the FACS analysis. The result showed that ACRO's Star Staining FITC-Labeled Human BCMA (Cat. No. BCA-HF2H3) and CD19 (Cat. No. CD9-HF2H3) protein have a much higher binding activity than that of the other competitor.

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Alexa Fluor 647-Labeled Human Mesothelin (296-580)

The activity of Alexa Fluor 647-Labeled Human Mesothelin (296-580), His Tag (Cat. No. MSN-HA2H5) was higher than other Competitor.

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Alexa Fluor 488-Labeled Human Mesothelin / MSLN (296-580) Protein

The activity of Alexa Fluor 488-Labeled Human Mesothelin (296-580), His Tag (Cat. No. MSN-HA2H9) was higher than other Competitor.

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Star Staining PE-labeled 和APC-labeled CD19蛋白产品经流式验证可以高灵敏度检测CD19 CAR表达。
PE-CD19  灵敏度FACS验证数据图

Anti-CD19 CAR-293 cells were stained with PE-Labeled Human CD19 (20-291) Protein (Cat. No. CD9-HP2H5), His Tag star staining and negative control protein respectively, PE signal was used to evaluate the binding activity.

APC-CD19  灵敏度FACS验证数据图

Anti-CD19 CAR-293 cells were stained with APC-Labeled Human CD19 (20-291) Protein (Cat. No. CD9-HA2H9), His Tag star staining and negative control protein respectively, APC signal was used to evaluate the binding activity.

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极低非特异背景

Star Staining 系列产品经未转染CAR的PBMC细胞双染批检测验证,无非特异背景问题,特异性显著高于竞品,保证CAR表达检测结果的特异性和准确性,适用于CAR-T临床样本分析。
BCMA-PBMC  FACS验证数据图

Non-specific binding to non-transduced PBMCs between PE-Labeled Human BCMA Protein of Acro and competitor. 5e5 of non-transduced PBMCs were stained with PE -Labeled Human BCMA Protein and anti-CD3 antibody, washed and then analyzed with FACS. FITC signal was used to evaluate the expression of CD3+ T cells in non-transduced PBMCs, and PE signal was used to evaluate the non-specific binding activity to non-transduced PBMCs.

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BCMA PBMC 验证对比图

Non-specific binding to non-transduced PBMCs between FITC-Labeled Human BCMA Protein of Acro and competitor. 5e5 of non-transduced PBMCs were stained with FITC-Labeled Human BCMA Protein and anti-CD3 antibody, washed and then analyzed with FACS. PE signal was used to evaluate the expression of CD3+ T cells in non-transduced PBMCs, and FITC signal was used to evaluate the non-specific binding activity to non-transduced PBMCs.

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可保持蛋白天然活性

Star Staining FITC-labeled Human BCMA产品可保持天然蛋白活性。
FACS验证结果显示标记FITC前后BCMA蛋白天然活性保持不变
BCMA活性验证FACS数据图

Binding activity of the Human BCMA before and after FITC labeling was evaluated in the above FACS analysis. The result shows that FITC-Labeled BCMA (Cat. No. BCA-HF2H3) and unconjugated Human BCMA have almost the same level of binding activity.

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SPR验证结果显示标记FITC前后BCMA蛋白的亲和力水平无差异
BCMASPR亲和力验证数据图
FITC-BCMASPR亲和力验证数据图

Binding affinity of the Human BCMA before and after FITC labeling was evaluated in the above SPR analysis (Biacore T200). The result shows that FITC-Labeled (Cat. No. BCA-HF2H3) and unconjugated Human BCMA, His Tag have almost the same level of affinity.

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高批间一致性

不同荧光标记产品的不同批次蛋白活性可保持一致,具有高批间一致性,以确保长期用于CAR-T细胞质控和临床样本分析中检测结果的稳定性和可靠性。
FITC-BCMA批间一致性FACS验证数据图
AF555-BCMA批间一致性FACS验证数据图
PE-BCMA批间一致性FACS验证数据图

Binding activity of different lots of FITC-Labeled Human BCMA (Cat. No. BCA-HF2H3) , AF555-Labeled Human BCMA (Cat. No. BCA-HA2H7) and PE-Labeled Human BCMA (Cat. No. BCA-HP2H7) against anti-BCMA CAR-293 cells was evaluated by flow cytometry. The result shows very high batch-to-batch consistency.

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高稳定性

高稳定性:25℃条件下加速48h和反复冻融三次,依然可保持较高的生物活性,显示出蛋白的高度稳定性,可放心储存和使用。
PE-BCMA高稳定性FACS验证数据图
FITC-BCMA高稳定性FACS验证数据图

PE-Labeled Human BCMA (Cat. No. BCA-HP2H7) and FITC-Labeled Human BCMA (Cat. No. BCA-HF2H3) at 25℃ for 48 hours and freeze-throw cycles are stable without performance reduction.

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参考文献

  • [1] Abbasov ME, Kavanagh ME, Ichu TA, et al. A proteome-wide atlas of lysine-reactive chemistry [published online ahead of print, 2021 Sep 9]. Nat Chem. 2021;10.1038/s41557-021-00765-4.
  • [2] Xu L, Kuan SL, Weil T. Contemporary Approaches for Site-Selective Dual Functionalization of Proteins. Angew Chem Int Ed Engl. 2021;60(25):13757-13777.
  • [3] Devabhaktuni A, Lin S, Zhang L, et al. TagGraph reveals vast protein modification landscapes from large tandem mass spectrometry datasets. Nat Biotechnol. 2019;37(4):469-479.
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