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STAT通路与疾病发生

信号通路(Signal pathway)是指能将细胞外的分子信号经细胞膜传入细胞内发挥效应的一系列酶促反应通路。一个信号通路的构成主要包括三部分元件,即受体(receptor)与配体(ligand)、蛋白激酶(kinase)、转录因子。在信号传导过程中,配体与细胞膜表面或细胞内受体结合,蛋白激酶通过磷酸化改变下游蛋白构象从而对一系列下游蛋白进行调节并将信号级联放大,最终通过转录因子对基因转录进行调节最终产生一系列细胞的综合应答。 信号传导过程是细胞对外界刺激产生反应并最终引发特异性生物学效应的有效方式。几十年的基础研究逐渐解释了许多在癌症、心血管疾病、自身免疫性疾病等疾病发生与发展过程中起关键作用的信号通路。同时,针对信号通路的药物筛选与开发也成为了疾病免疫治疗的新思路。
JAK-STAT信号传送途径是一个进化保守的信号级联反应,介导对细胞因子和生长因子的反应,使得细胞外的化学信号跨越细胞膜并将信息传送到细胞核内DNA上的基因启动子,最终引起细胞中DNA转录与活性水平发生改变,是除了第二信使系统外最重要的信号途径。JAK-STAT系统由三个主要部分组成:受体、JAK激酶、信号转导及转录激活蛋白(STAT)。
ACRO基于充分的信号通路研究,结合靶向信号通路的药物作用机制,开发了经典信号通路JAK/STAT相关产品,提供包括细胞因子、生长因子等信号分子、细胞膜表面受体及配体、蛋白激酶、转录因子等靶点蛋白,同时拓展有包括预偶联磁珠、抑制剂筛选试剂盒、报告基因细胞系等更多产品类型,力求为信号通路研究及靶向信号通路的药物开发提供一站式高效解决方案。
热门通路
JAK/STAT5
JAK/STAT5

信号通路:肺和肠道上皮细胞和角质形成细胞收到刺激后释放TSLP,与其受体TSLPR结合形成二聚体后募集IL-7Rα,形成三元TSLPR-TSLP-IL-7Rα复合物,进而通过JAK和STAT5启动促炎信号。

相关疾病:过敏性疾病、慢性炎症、自身免疫性疾病、癌症等。

热门产品:TSLP、TSLPR、IL-7 R alpha、IL-7 R alpha & TSLP R Hot、TSLP抑制剂筛选试剂盒、TSLPR报告基因细胞系New

JAK/STAT3
JAK/STAT3

信号通路:JAK激酶是许多细胞因子、生长因子及干扰素的重要信号传感器。在某些应激反应条件下,一些细胞因子与相应的受体结合,诱导JAK磷酸化,从而使STAT3活化,促使STAT3的二聚化、核易位、与特定的DNA序列结合,行使基因转录调控的功能。

相关疾病:肿瘤、免疫类疾病、感染类疾病等。

热门产品:细胞因子、生长因子、JAK1 New、STAT3New、EGFR预偶联磁珠、EGFR报告基因细胞系、STAT3报告基因细胞系。

JAK/STAT5
  • 免疫/结合验证——靶点蛋白&受体
  • 抗体/抑制剂筛选——抑制剂筛选试剂盒
  • 细胞水平功能验证——报告基因细胞系

靶点蛋白:

种属齐全:覆盖Human、Mouse、Cynomolgus不同种属;
标签选择丰富:提供His、His & Avi、Fc、mFc、Fc & Fc不同产品形式;
模拟天然构象:除TSLP、TSLP R、IL-7 R外,特别推出行业新品TSLP R & IL7 R异源二聚体,真实模拟天然构象;
纯度及活性保证:纯度经SDS-PAGE、MALS验证,活性经ELISA、SPR、BLI等多平台验。
产品列表
分子 货号 物种 产品描述 订购/预购
TSLP TSP-C52H4 Cynomolgus Cynomolgus TSLP (R127A, R130S) Protein, His Tag

订购

TSP-C52H8 Cynomolgus Cynomolgus TSLP Protein, His Tag

订购

TSP-C82E3 Cynomolgus Biotinylated Cynomolgus TSLP Protein, His,Avitag™

订购

TSP-H5255 Human Human TSLP (R127A, R130A) Protein, Mouse IgG2a Fc Tag

订购

TSP-H52Ha Human Human TSLP (R127A, R130A) Protein, His Tag (MALS verified)

订购

TSP-H52Hb Human Human TSLP Protein, His Tag

订购

TSP-H82E0 Human Biotinylated Human TSLP (R127A, R130A) Protein, His,Avitag™ (MALS verified)

订购

TSP-H82Eb Human Biotinylated Human TSLP Protein, His,Avitag™

订购

TSP-M52H8 Mouse Mouse TSLP Protein, His Tag

订购

TSP-M82E3 Mouse Biotinylated Mouse TSLP Protein, His,Avitag™

订购

TSP-R52H3 Rat Rat TSLP Protein, His Tag

订购

TSLP R TSR-H525a Human Human TSLP R Protein, Fc Tag

订购

IL-7 R alpha & TSLP R ILR-H5255 Human Human IL-7 R alpha&TSLP R Heterodimer Protein, Fc Tag&Fc Tag (MALS verified)

订购

ILR-H525x Human Human IL-7 R alpha&TSLP R Heterodimer Protein, Fc Tag&Fc Tag

订购

IL-7 R alpha IL7-H5258 Human Human IL-7 R alpha / CD127 Protein, Mouse IgG2a Fc Tag

订购

IL7-H82F8 Human Biotinylated Human IL-7 R alpha / CD127 Protein, Fc,Avitag™

订购

ILA-H525a Human Human IL-7 R alpha / CD127 Protein, Fc Tag

订购

结合活性验证——TSLP蛋白及其受体
TSLP R & IL7 R异源二聚体纯度经MALS验证>85%

The purity of Human IL-7 RA&TSLP R Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. ILR-H5255) is greater than 95% verified by SDS-PAGE. The purity of the heterodimer is more than 85% and the molecular weight of this protein is around 125-140 kDa verified by SEC-MALS.

Protocol

TSLP R & IL7 R异源二聚体可用于验证抗体与TSLP结合活性

Serial dilutions of Monoclonal Anti-Human TSLP Antibody, Human IgG2 were added into Human IL-7 RA&TSLP R Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. ILR-H5255): Biotinylated Human TSLP, His,Avitag (Cat. No. TSP-H82Eb) binding reactions. The half maximal inhibitory concentration (IC50) is 1.09688 μg/mL (Routinely tested).

Protocol

抑制剂筛选试剂盒:

快速,高通量,可在2h内完成90+样本检测;
严格指控,保证试剂盒批间差及精密度均<10%;
预包被模拟天然构象的TSLPR和IL-7R异源二聚体,提高筛选成功率。
产品列表
货号 产品描述 订购/预购
EP-129 TSLP[Biotinylated]:IL-7Rα & TSLP R Inhibitor Screening ELISA Kit

订购

抗体/抑制剂筛选——抑制剂筛选试剂盒

Serial dilutions of Anti-TSLP Neutralizing antibody (Catalog # EP129-C03) (1:1 serial dilution, from 8 μg/mL to 0.0156 μg/mL (55.317-0.108 nM)) was added into IL-7 R alpha & TSLP R: TSLP-Biotin binding reactions. The assay was performed according to the above-described protocol. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).

Protocol

功能细胞系:

代次稳定性好,10-26代传代稳定性经验证;
检测窗口大, 适用不同药效筛选;
反应信号强,确保高灵敏度;
基于明确MOA设计,可用于药物开发过程中的细胞功能性验证实验及CMC质控放行。
产品列表
货号 产品描述 订购/预购
CHEK-ATF045 TSLPR (Luc) HEK293 Reporter Cell

订购

细胞水平功能验证——报告基因细胞系
细胞性质验证

Co-expression analysis of human TSLPR and IL7Rα on TSLPR (Luc) HEK293 Reporter Cell by FACS. Cell surface staining was performed on TSLPR (Luc) HEK293 Reporter Cell or negative control cell using PE-labeled anti-TSLPR antibody and FITC-labeled anti-IL7Rα antibody.

Protocol

Response to human TSLP protein (RLU). The TSLPR (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human TSLP protein (Cat. No. TSP-H52Hb). The EC50 was approximately 0.1392 μg/mL.

Protocol

Response to human TSLP protein (Fold). The TSLPR (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human TSLP protein (Cat. No. TSP-H52Hb). The max induction fold was approximately 45.15.

Protocol

代次稳定性验证

Passage stability analysis of receptors expression by FACS. Flow cytometry surface staining of human TSLPR and IL7Rα on TSLPR (Luc) HEK293 Reporter Cell demonstrates consistent mean fluorescent intensity across passage10-26. (A) Human IL7Rα expression analysis. (B) Human TSLPR expression analysis.

Protocol

Passage stability analysis by Signaling Bioassay. The continuously growing TSLPR (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human TSLP protein. Human TSLP protein stimulated response demonstrates passage stabilization (fold induction and EC50) across passage 10-26.

Protocol

应用案例

Inhibition of human TSLP protein-induced reporter activity by anti-human TSLP neutralizing antibody. This reporter cell was incubated with serial dilutions of antibodies in the presence of human TSLP protein (Cat. No. TSP-H52Hb) with a final concentration of 0.3 μg/mL. The EC50 of anti-human TSLP neutralizing antibody is approximately 0.55 μg/mL.

Protocol

JAK/STAT3
  • 免疫/结合验证——靶点蛋白&受体
  • 抗体/抑制剂筛选——预偶联磁珠
  • 细胞水平功能验证——报告基因细胞系

靶点蛋白:

胞内胞外靶点丰富:覆盖STAT3经典信号通路内外靶点蛋白,包括EGF、EGR R、IL-6、IL-6 R alpha、JAK1、STAT3等;
种属选择多样:覆盖Human、Mouse、Cynomolgus不同种属;
标签选择丰富:提供His、His & Avi、Fc、mFc、Fc & Fc不同产品形式;
纯度活性保证:纯度经SDS-PAGE、MALS验证>90%,与降解剂结合活性经BLI、SPR等实验方法验证。
产品列表
分子 货号 物种 产品描述 订购/预购
EGF EGF-H5119 Human Human EGF Protein, Tag Free

预购

EGF-H525b Human Human EGF Protein, Mouse IgG2a Fc Tag

订购

EGF-H52H3 Human Human EGF Protein, His Tag

订购

EGF-M5265 Mouse Mouse EGF Protein, Fc Tag

订购

EGF R EGR-C5252 Rhesus macaque Rhesus macaque EGF R Protein, Fc Tag (MALS verified)

订购

EGR-C52H1 Rhesus macaque Rhesus macaque EGF R Protein, His Tag

订购

EGR-H5222 Human Human EGF R Protein, His Tag (MALS verified)

订购

EGR-H522a Human Human EGF R Protein, His Tag, low endotoxin (MALS verified)

订购

EGR-H5252 Human Human EGF R Protein, Fc Tag (MALS verified)

订购

EGR-H5259 Human Human EGF R Protein, Llama IgG2b Fc Tag, low endotoxin

订购

EGR-H82E3 Human Biotinylated Human EGF R Protein, His,Avitag™ (MALS verified)

订购

EGR-H82F8 Human Biotinylated Human EGF R Protein, Fc,Avitag™ (MALS verified)

订购

EGR-HF256 Human FITC-Labeled Human EGF R Protein, Fc Tag

订购

EGR-HF2H5 Human FITC-Labeled Human EGF R Protein, His Tag DMF Filed

订购

EGR-HP2E3 Human PE-Labeled Human EGF R Protein, His Tag (Site-specific conjugation)

订购

EGR-M5224 Mouse Mouse EGF R Protein, His Tag

订购

EGR-R52H7 Rabbit Rabbit EGF R Protein, His Tag

订购

IL-6 IL6-C5249 Cynomolgus Cynomolgus IL-6 Protein, His Tag (MALS verified)

订购

IL6-H4218 Human Human IL-6 Protein, premium grade

订购

IL6-H8218 Human Biotinylated Human IL-6 Protein, epitope tag free, ultra sensitivity (primary amine labeling)

订购

IL6-H82Q9 Human Biotinylated Human IL-6 Protein, Avitag™,His Tag

订购

IL6-M5245 Mouse Mouse IL-6 Protein, His Tag

订购

IL6-M82Q7 Mouse Biotinylated Mouse IL-6 Protein, Avitag™,His Tag

订购

IL-6 R alpha CD6-H82E8 Human Biotinylated Human IL-6 R alpha / CD126 Protein, Avitag™,His Tag

订购

ILR-H4223 Human Human IL-6 R alpha / CD126 Protein, His Tag

订购

ILR-H5259 Human Human IL-6 R alpha / CD126 Protein, Fc Tag

订购

ILR-H82F9 Human Biotinylated Human IL-6 R alpha / CD126 Protein, Fc,Avitag™

订购

ILR-M82E9 Mouse Biotinylated Mouse IL-6 R alpha / CD126 Protein, His,Avitag™

订购

gp130 ILT-H52H2 Human Human gp130 / CD130 / IL-6 R beta Protein, His Tag

预购

ILT-M5252 Mouse Mouse gp130 / CD130 / IL-6 R beta Protein, Fc Tag

订购

ILT-M52H1 Mouse Mouse gp130 / CD130 / IL-6 R beta Protein, His Tag

订购

结合活性验证——TSLP蛋白及其受体
人源STAT3蛋白(Cat. No. ST3-H5149) 纯度经MALS验证>90%

The purity of Human STAT3, His Tag (Cat. No. ST3-H5149) is more than 95% verified by SDS-PAG and greater than 90% verified by SEC-MALS. The molecular weight of this protein is around 60-75 kDa.

Protocol

人源STAT3蛋白与其降解剂结合活性经SPR验证

Human STAT3, His Tag (Cat. No. ST3-H5149) immobilized on CM5 Chip can bind STAT3 degrader with an affinity constant of 44.6 nM as determined in a SPR assay (Biacore 8K) (QC tested).

Protocol

预偶联磁珠:

采用生物素化蛋白-链霉亲和素磁珠系统,饱和浓度抗原,保证充分包被;
磁珠粒径均匀、比表面积大,捕获效率高,结果重复性好。
产品列表
货号 产品描述 订购/预购
MBE-K012 Human EGFR-coupled Magnetic Beads

订购

抗体/抑制剂筛选——预偶联磁珠

Immobilized 35 μg EGFR protein to 1 mg Beads, can bind the Anti-EGFR Antibody (Cetuximab) with an EC50 of 0.737 μg/mL (QC tested).

Protocol

功能细胞系:

基于明确MOA设计,提供EGFR/STAT3和IL-6/JAK/STAT3通路报告基因细胞系;
代次稳定性好,可稳定传代10-20代;
检测窗口大, 适用不同药效筛选;
反应信号强,确保高灵敏度;
可用于靶向该信号通路的小分子抑制剂与抗体药筛选及细胞功能验证实验。
产品列表
货号 产品描述 订购/预购
CHEK-ATF049 EGFR (Luc) HEK293 Reporter Cell

订购

CHEK-ATF047 STAT3 (Luc) HEK293 Reporter Cell

订购

细胞水平功能验证——报告基因细胞系
细胞性质验证

Expression analysis of human EGFR on EGFR (Luc) HEK293 Reporter Cell by FACS. Cell surface staining was performed on EGFR (Luc) HEK293 Reporter Cell or negative control cell using PE-labeled anti-EGFR antibody.

Protocol

Response to human EGF protein (RLU). The EGFR (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human EGF protein (Cat. No. ST3-H5149). The EC50 was approximately 56.23 ng/mL.

Protocol

Response to human EGF protein (Fold). The EGFR (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human EGF protein (Cat. No. ST3-H5149). The max induction fold was approximately 56.

Protocol

代次稳定性验证

Passage stability analysis of receptors expression by FACS. Flow cytometry surface staining of human TSLPR and IL7Rα on TSLPR (Luc) HEK293 Reporter Cell demonstrates consistent mean fluorescent intensity across passage10-26. (A) Human IL7Rα expression analysis. (B) Human TSLPR expression analysis.

Protocol

Passage stability analysis by Signaling Bioassay. The continuously growing TSLPR (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human TSLP protein. Human TSLP protein stimulated response demonstrates passage stabilization (fold induction and EC50) across passage 10-26.

Protocol

应用案例

Inhibition of human EGF protein-induced reporter activity by human EGFR small molecule inhibitor. This reporter cell was incubated with serial dilutions of inhibitors in the presence of human EGF protein (Cat. No. ST3-H5149) with a final concentration of 50 ng/mL. The EC50 of human EGFR small molecule inhibitor (Erlotinib) was approximately 0.01 μM.

Protocol

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