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VEGF蛋白:切断肿瘤的供养通道

背景
血管内皮生长因子(Vascular endothelial growth factor, VEGF) 除了具有促进血管新生和再生等重要作用外,还具有免疫调节、神经保护、组织修复等多种功能。VEGF主要与受体VEGFR相互结合发挥功能。研究表明VEGF参与许多疾病的发生,在多种恶性肿瘤中均有表达,特别在肿瘤组织血管增生丰富的部位,因此成为药物开发的主要靶点。针对VEGF有100多种抗体药处于各种研发阶段,其中多款单抗药物已经上市,针对这一靶点的双抗药也在积极开发中。覆盖包括肿瘤、视觉性疾病以及心血管疾病等多种类型,具有极大潜力。
ACROBiosystems开发了多种VEGF家族蛋白产品。覆盖不同种属、不同亚型、不同标签或者无标签的蛋白产品,满足不同的实验需求。
产品列表
分子 货号 host 产品描述 结构
VEGF165VE5-H4210HEK293ActiveMax® Human VEGF165 Protein, Tag Free (MALS verified)HOT
VEGF165VE5-H5248HEK293Human VEGF165 Protein, His TagHOT
VEGF165VE5-H8210HEK293Biotinylated Human VEGF165, epitope tag free, ultra sensitivity (primary amine labeling)HOT
VEGF165VE5-H82Q0HEK293Biotinylated Human VEGF165 Protein, His,Avitag™HOT
VEGF121VE1-H4213HEK293ActiveMax® Human VEGF121 Protein, Tag FreeHOT
VEGF121VE1-H5246HEK293Human VEGF121 Protein, His TagHOT
VEGF121VE1-H82E7HEK293Biotinylated Human VEGF121 Protein, Avitag™,His TagHOT
VEGF-CVEC-H4225HEK293Human VEGF-C / Flt4-L Protein, His TagHOT
VEGF110VE0-H5212HEK293ActiveMax® Human VEGF110 Protein, Tag Free (HPLC-verified)
VEGF120VE0-M4211HEK293ActiveMax® Mouse VEGF120 Protein, Tag Free
VEGF120VE0-M82Q2HEK293Biotinylated Mouse VEGF120 Protein, His,Avitag™
VEGF164VE4-M4216HEK293ActiveMax® Mouse VEGF164 Protein, Tag Free (HPLC-verified)
VEGF164VE4-M82Q3HEK293Biotinylated Mouse VEGF164 Protein, His,Avitag™
VEGF R1VE1-H5220HEK293Human VEGF R1 / Flt-1 Protein, His Tag
VEGF R1VE1-H82E3HEK293Biotinylated Human VEGF R1 / Flt-1 Protein, His,Avitag™
VEGF R1VE1-M5256HEK293Mouse VEGF R1 / Flt-1 Protein, Mouse IgG2a Fc Tag, low endotoxin
VEGF R1VE1-R5257HEK293Rhesus macaque VEGF R1 / Flt-1 Protein, Mouse IgG2a Fc Tag, low endotoxin
VEGF R2KDR-H5227HEK293Human VEGF R2 / KDR Protein, His Tag (MALS verified)
VEGF R2KDR-H5280HEK293Human VEGF R2 / KDR Protein, Strep Tag (MALS verified)
VEGF R2KDR-H82E5HEK293Biotinylated Human VEGF R2 / KDR Protein, Avitag™,His Tag
VEGF R2VE2-C52H3HEK293Rhesus macaque VEGF R2 / KDR Protein, His Tag
VEGF R2VE2-HF254HEK293FITC-Labeled Human VEGF R2 / KDR Protein, His Tag
VEGF R2VE2-M5258HEK293Mouse VEGF R2 / KDR Protein, Mouse IgG2a Fc Tag, low endotoxin
VEGF R3FL4-H5251HEK293Human VEGF R3 / FLT4 Protein, Fc Tag
VEGF R3FL4-H82E1HEK293Biotinylated Human VEGF R3 / FLT4 Protein, His,Avitag™
VEGF R3FL4-M5251HEK293Mouse VEGF R3 / FLT4 Protein, Mouse IgG2a Fc Tag, low endotoxin
VEGF-BVE6-H5225HEK293Human VEGF-B Protein, His Tag
VEGF-DVED-H5228HEK293Human VEGF-D Protein, His Tag

>>>如果您希望ACRO开发更多类型的VEGF的产品或者有定制化需求,请点此 联系我们

>>> 了解更多热门双特异性抗体热门靶点

验证数据
高纯度经MALS验证
The purity of ActiveMax® Human VEGF165, Tag Free (MALS verified) (Cat. No. VE5-H4210) was more than 95% in HP-SEC, and the molecular weight of this protein is around 40-55 kDa verified by SEC-MALS.
生物活性经ELISA&SPR&BLI&细胞实验等验证
>>ELISA

Immobilized Human VEGF R3, Fc Tag (Cat. No. FL4-H5251) at 5 μg/mL (100 μL/well) can bind Human VEGF-C, His Tag (Cat. No. VEC-H4225) with a linear range of 2-20 ng/mL (QC tested).

Protocol

>>SPR

Immobilized Biotinylated Human VEGF165, His,Avitag (Cat. No. VE5-H82Q0) on CM5 Chip via Streptavidin, can bind Avastin with an affinity constant of 0.417 nM as determined in SPR assay (Biacore T200) (Routinely tested).

Protocol

>>BLI

Loaded Avastin (Bevacizumab) on AHC Biosensor, can bind Human VEGF165, His Tag (Cat. No. VE5-H5248) with an affinity constant of 0.448 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Protocol

>>细胞实验

ActiveMax® Human VEGF165, Tag Free (MALS verified) (Cat. No. VE5-H4210) stimulates proliferation of human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is 4.216-9.281 ng/mL (Routinely tested).

Protocol

Inhibition assay shows that the proliferation effect of ActiveMax® Human VEGF165, Tag Free (MALS verified) (Cat. No. VE5-H4210) is inhibited by increasing concentration of anti-VEGF mAb (Avastin). The concentration of VEGF165 used is 20 ng/mL. The ED50 is 0.065-0.229 μg/mL (Routinely tested).

Protocol

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