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更加灵敏检测Anti-CD19 (FMC63) CAR的特异性抗体

抗体特性经Flow Cytometry验证并免费提供Protocol

背景
CD19是目前CAR-T细胞治疗领域中应用最广泛的靶点,大量研究表明CD19是治疗B-ALL,CLL和B细胞淋巴瘤有效且安全的靶点。FMC63是一种靶向人CD19的鼠单克隆抗体。到目前为止,大多数报道的CART19试验项目均是以FMC63抗体序列为基础构建的CAR-T,包括FDA批准上市的两种Anti-CD19 CAR-T细胞药物Kymriah和Yescarta。根据2018年中检院颁布的《CAR-T细胞治疗产品质量控制检测研究及非临床研究考虑要点》,CAR转染阳性率的检测应采用流式细胞法,推荐使用具有更好专属性的针对抗原结合部位的抗原蛋白或抗独特型抗体进行检测。
然而市面上现存的CD19抗原大多灵敏度不够而无法满足检测需求,ACROBiosystems开发的高亲和力人CD19蛋白在检测Anti-CD19 CAR阳性表达率方面显现出了高特异性、高灵敏度的特性,被广泛用于Anti-CD19 CAR-T开发过程中慢病毒包装后的滴度测定、CAR基因转染/转导效率的检测和Anti-CD19 CAR-T细胞的质控放行中,获得了业内的广泛认可。
高亲和力人CD19蛋白之后,ACROBiosystems持续发力推出特异性靶向FMC63 scFv抗原识别表位的抗独特型抗体,进一步丰富了FMC63 CAR的检测手段。此抗体同高亲和力人CD19蛋白一样具有灵敏度高,特异性好的特点,经充分验证可适用于Flow Cytometry (FCM)方法检测Anti-CD19 (FMC63) CAR的表达。
- 专为细胞分选和细胞培养应用而设计;
- 产品在无动物条件下生产,整个生产过程中不使用任何动物源性成分;
- 经严格的质量控制,包括无菌、支原体、内毒素测试等,保证安全性;
- 产品性能经全面验证,以适用于早期临床前阶段细胞培养或其他相关应用。
产品列表
Molecule Cat. No. Species Source Product Description
FMC63 FM3-AY54P1 Mouse HEK293 APC-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) (Site-specific conjugation)
FM3-AY54A1 Mouse HEK293 APC-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) (Site-specific conjugation) (0.03% Proclin)
FM3-AM534 Mouse HEK293 Alexa Fluor™ 647-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45)Star Staining
FM3-HPY53 Mouse HEK293 PE-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) (Site-specific conjugation) (Preservative free)
FM3-PY54G0 Mouse HEK293 PE-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45), premium grade (Site-specific conjugation)
FM3-PY54A2 Mouse HEK293 PE-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) (Site-specific conjugation) (0.03% Proclin)
CD9-M52Hb Mouse HEK293 FMC63 scFv, His Tag (SPR verified)
FM3-Y45P1 Mouse HEK293 Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) (HEK293)
FM3-Y45 Mouse Hybridoma Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45)
FM3-Y45A1 Mouse HEK293 Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) (Carrier-free) (recommended for ADA assay)
FM3-FY45G0 Mouse HEK293 FITC-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45), premium grade
FM3-FY45P1 Mouse HEK293 FITC-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) (HEK293)
FM3-FY45 Mouse Hybridoma FITC-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) New
CD9-BV4324b Mouse HEK293 Biotinylated FMC63 scFv, Fc, (MALS verified)
FM3-BY54 Mouse HEK293 Biotinylated Monoclonal Anti-FMC63 Antibody, Mouse IgG1, (Y45)
MBS-C008 Mouse ActiveMax® Anti-FMC63 Antibody μBeads, premium grade (for cells)
FM3-S93 Rabbit - Rabbit Anti-Mouse FMC63 Polyclonal Antibody (recommended for ADA assay) (MALS verified)
RAB-P001 Rabbit Anti-CD19 (FMC63) CAR Immunogenicity ELISA Kit
产品特点
能够高特异无背景检测Anti-CD19 (FMC63) CAR-T临床样本
Detection of Anti-CD19 (FMC63) CAR Expression

2e5 of FMC63 scFv-based anti-CD19 CAR-293 cells were stained with 100 µL of the working solution of Monoclonal AntiFMC63 scFv Antibody, Mouse IgG1 (Cat. No. FM3-Y45) and isotype control respectively, washed and then followed by PE anti-mouse IgG1 Antibody and analyzed with flow cytometry (QC tested).

Protocol

Assessment of Non-specific Binding to 293 Cells

Non-specific binding of Monoclonal Anti-FMC63 scFv Antibody (Cat. No. FM3-Y45) to non-transfected 293 cells was determined by flow cytometry. The data showed that Anti-FMC63 scFv Antibody didn’t bind to non-transfected 293 cells.

Protocol

Assessment of Non-specific Binding to PBMCs

Non-specific binding of Monoclonal Anti-FMC63 scFv Antibody (Cat. No. FM3-Y45) to non-transfected human PBMCs was determined by flow cytometry. The data showed that Anti-FMC63 scFv Antibody didn’t bind to non-transfected human PBMCs.

Protocol

能够更灵敏且特异地识别Anti-CD19 (FMC63) CAR的抗原结合位点
Binding Activity and Specificity Measured by ELISA

Immobilized FMC63 scFv, His Tag at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Clone Y45) with a linear range of 1-19 ng/mL. Anti-DNP antibody, mouse IgG1 (Cat. No. DNP-M1) was used as an isotype control (QC tested).

Protocol

Neutralizing Activity Measured by ELISA

ELISA analysis shows that the binding of Human CD19, Fc Tag (Cat. No. CD9-H5251) to FMC63 scFv, His Tag was inhibited by increasing concentration of Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Clone Y45). The concentration of Human CD19, Fc Tag used is 5 μg/mL (100 μL/well). The IC50 is 0.084 μg/mL (Routinely tested).

Protocol

High Affinity Determined by SPR

Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Cat. No. FM3-Y45) captured on CM5 chip via anti-mouse antibodies surface can bind FMC63 scFv with an affinity constant of 1.08 nM as determined in a SPR assay

Protocol

相关产品
高亲和力CD19蛋白产品列表
Molecule Cat. No. Product Description
CD19 XL002023002
CD9-HA2H7 Alexa Fluor™ 555-Labeled Human CD19 (20-291) Protein, His TagStar Staining
CD9-HA2H5 APC-Labeled Human CD19 (20-291) Protein, His Tag (Site-specific conjugation)
CD9-HA2H9 APC-Labeled Human CD19 (20-291) Protein, His TagStar Staining
CD9-HA2H3 Alexa Fluor™ 488-Labeled Human CD19 (20-291) Protein, His TagStar Staining
CD9-HA2H6 Alexa Fluor™ 647-Labeled Human CD19 (20-291) Protein, His TagStar Staining
CD9-HP2H5 PE-Labeled Human CD19 (20-291) Protein, His TagStar Staining
CD9-HP2H3 PE-Labeled Human CD19 (20-291) Protein, His Tag (Site-specific conjugation) New
CD9-HF2H2 FITC-Labeled Human CD19 (20-291) Protein, His Tag
CD9-HF2H3 FITC-Labeled Human CD19 (20-291) Protein, His TagStar Staining
CD9-HF251 FITC-Labeled Human CD19 (20-291) Protein, Fc Tag
CD9-H8259 Biotinylated Human CD19 Protein, Fc Tag, ultra sensitivity (primary amine labeling)
MBS-C002 ActiveMax® Human CD19 μBeads, premium grade (for cells)
CD9-H82F6 Biotinylated Human CD19 (20-291) Protein, Fc,Avitag™, premium grade
CD9-H82E9 Biotinylated Human CD19 (20-291) Protein, His,Avitag™, premium grade New
MBS-K005 Human CD19-coupled Magnetic Beads
CHEK-ATS056 HEK293/Human Anti-CD19 Stable Cell Line
CHEK-ATP003 HEK293/Human CD19 Stable Cell Line
CD9-H5258 Human CD19 (20-291) Protein, Mouse IgG2a Fc Tag (MALS verified)
CD9-H5251 Human CD19 (20-291) Protein, Fc Tag, premium grade
CD9-H52H2 Human CD19 (20-291) Protein, His Tag
CD9-H5259 Human CD19 (20-291) Protein, Fc Tag, low endotoxin
CD9-M52H8 Mouse CD19 Protein, His Tag
CD9-C5251 Cynomolgus / Rhesus macaque CD19 (20-292) Protein, Fc Tag
CD9-C5221 Cynomolgus / Rhesus macaque CD19 (20-292) Protein, His Tag
CD9-R52H6 Rat CD19 Protein, His Tag
CD9-R52H3 Rabbit CD19 Protein, His Tag
HGS-S259 Recombinant Monoclonal Anti-CD19 Antibody, Rabbit (4A6)
CD9-C52H4 Canine CD19 Protein, His Tag
更多CAR-T相关产品
> 点击查看更多CAR-T靶点信息 > 点击查看更多CD19详细信息——高亲和力CD19蛋白,专为检测CD19-CAR-T而设计
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