resDetect™ T7 RNA Polymerase ELISA Kit (Residue Testing)

背景（Background）

The biology and function of IFN-γ has been extensively reviewed in the literature. Cytometric Bead Array (CBA) assays provide a method of capturing a soluble analyte or set of analytes with beads of known size and fluorescence, making it possible to detect analytes using flow cytometry.

应用说明（Application）

T7 RNA Polymerase ELISA Kit (Residue Testing)was developed for the detection and quantitative determination of T7 RNA Polymerase in mRNA preparation processing where T7 RNA Polymerase is used as an row agent.

It is for research use only.

存储（Storage）

The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

原理（Assay Principles）

This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of T7 RNA polymerase. The kit consists of Pre-coated Anti-T7 RNA polymerase Antibody Microplate and T7 RNA polymerase Standard and Biotin-Anti-T7 RNA polymerase Antibody and Streptavidin-HRP and buffers.

Your experiment will include 6 simple steps:

a) Bring all reagents to room temperature(20℃-25℃) before use.

b) Add your sample to the plate and take the T7 RNA polymerase as standard. The samples and standard are diluted by Dilution Buffer.

c) Add the Biotin-Anti-T7 RNA polymerase Antibody diluted by Dilution Buffer to the plate.

d) Wash the plate and add the Streptavidin-HRP diluted by Dilution Buffer to the plate.

e) Wash the plate and add TMB.

f) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated by the absorbance at 450 nm minus the absorbance at 630 nm to remove background disturbance before statistical analysis. The OD Value reflects the amount of bound enzyme.