免疫检查点蛋白

免疫检查点（Immune Checkpoint）通路包括共刺激蛋白（Costimulatory Proteins）和共抑制蛋白（Coinhibitory Proteins）。共刺激蛋白会传导信号促进对病原体的免疫反应。相反，共抑制蛋白则负向调节T淋巴细胞的活性，防止过度的炎症反应。近年来，免疫检查点蛋白因其在肿瘤发生和发展中的重要作用备受关注。通常，肿瘤细胞可以通过阻断免疫检查点通路来逃脱免疫系统的攻击。因此，研究者相信调控免疫检查点通路可使免疫系统重新激活，从而对抗肿瘤。值得一提的是，anti-CTLA4和anti-PD1单克隆药物已经在多种恶性肿瘤的临床试验中展现出不错的疗效，同时研究人员也在开发针对其他检查点蛋白的抗体药物，从而找到更好的治疗肿瘤的方法。

为了支持上述研究，ACROBiosystems公司开发了一系列免疫检查点蛋白系列产品，涵盖了已知的绝大部分免疫检查点分子。客户可以选择不同的标签（如Fc, His, Strep）或者无标签的蛋白产品。除了常用的人种属之外，我们还为动物实验提供小鼠、大鼠、兔、恒河猴（Rhesus Macaque）和食蟹猴（Cynomolgus）的蛋白。

产品特点

高质量的产品

标准规范的生产工艺加上严格的质量控制，保证了我们所有产品的高质量，我们的免疫检查点蛋白产品具有高纯度、高生物活性并且性能稳定。

SDS-PAGE和SEC-HPLC分析纯度大于90%
功能性ELISA验证生物活性
LAL方法检测内毒素水平小于1EU/µg

: 图2. 功能性ELISA分析human PD-L1（Cat. # PD1-H5258）结合PD1（Cat. # PD1-H5221）的生物活性

: 图1. SEC-HPLC分析Human PD-L1（Cat. # PD1-H5229）纯度大于95%；SDS-PAGE大于98%

生物素标记&重元素标记蛋白

我们也提供多种即时使用的生物素（Biotin）标记的检查点蛋白和重元素标记（Heavy Label）的蛋白。生物素标记蛋白采用自主研发并优化的标记技术，具有高生物活性和低批间差异，有助于快速建立检测平台；重元素标记蛋白可用于定量质谱分析。

抑制剂筛选试剂盒

此外，我们还开发了用于抑制剂筛选的试剂盒（Cat. # EP-101），可以快速并且高通量地筛选PD-1-PD-L1通路的抑制性抗体或小分子。

部分引用文献

HETEROCYCLIC COMPOUNDS AS IMMUNOMODULATORS

Authors: L Wu et al.

Journal: US20170107216 A1 2017

Product: Human PD-1（Cat. # PD1-H5257）, Human PD-L1（Cat. # PD1-H5229）

Application: HTRF Binding Assay

Download PDF:

Anti-pd-1 antibodies and methods of use thereof

A Novel PD-L1-targeting Antagonistic DNA Aptamer With Antitumor Effects

Hydrogel dual delivered celecoxib and anti-PD-1 synergistically improve antitumor immunity

Therapeutic monoclonal antibodies in human breast milk: a case study

客户感言

Vidal F.de la Cruz, Founder & Principal of Fomento Pharma, LLC

I have been very satisfied with Acrobiosystems’ products and customer service. Their technical support team has been extraordinarily proactive in making sure that our needs are being fully addressed.They always work closely with us to help us find the products that suited our research the best. This includes offering an array of samples for free for our preliminary testing. The demonstration of such awareness of subtle issues surrounding our research, was quite unexpected and highly impressive...

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Jean-François Jourdil, University Hospital of Grenoble

TNF-alpha immunocapture is easy to automate and offers a very specific sample preparation by isolating only the compound of interest. Furthermore the high specificity generates very low and robust MS/MS signal to noise ratios and has allowed us to gain a factor of ten in sensitivity. Thus our mass spectrometer which hitherto was not sensitive enough, can now detect levels of concentration of our therapeutic monoclonal antibody which are compatible with the clinical diagnosis.

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Adam D. Friedman, University of North Carolina at Chapel Hill

In my ten years of research experience, I have found that the protein products produced by Acros Biosystems are equal to none. They are exceptional in their purity and activity and, as such, have greatly enhanced the quality of my research. It has been a pleasure to find a great multitude of proteins, most with a variety of tags, all offered by a single company. In addition, the efficiency and cordiality of their customer service matches the excellence of their products, resulting in one very satisfied customer!

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