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 >  Protein>Her2 >HE2-HA2H5

Alexa Fluor™ 647-Labeled Human Her2 / ErbB2 Protein, His TagStar Staining

分子别名(Synonym)

ERBB2,CD340,HER-2,neu,HER2,MLN19,NEU,NGL,TKR1

表达区间及表达系统(Source)

Alexa Fluor 647-Labeled Human Her2, His Tag (HE2-HA2H5) is produced via conjugation of AF647 to Human Her2, His Tag with a new generation site-specific technology under Star Staining labeling platform. Human Her2, His Tag is expressed from human 293 cells (HEK293). It contains AA Thr 23 - Thr 652 (Accession # P04626-1 ).

Predicted N-terminus: Thr 23

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the C-terminus

The protein has a calculated MW of 83.9 kDa.

偶联(Conjugate)

AF647

Excitation Wavelength: 640 nm

Emission Wavelength: 672 nm

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

CAR阳性表达率检测(Evaluation of CAR expression)

FACS Analysis of Anti-Her2 CAR Expression

Her2 CAR_T

5e5 of anti-Her2 CAR-293 cells were stained with 100 μL of 1 μg/mL of Alexa Fluor 647-Labeled Human Her2, His Tag (Cat. No. HE2-HA2H5) and negative control protein respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). Alexa Fluor 647 signal was used to evaluate the binding activity (QC tested).

Protocol

FACS Analysis of Non-specific binding to PBMCs

Her2 CAR_T

5e5 of PBMCs were stained with Alexa Fluor 647-Labeled Human Her2, His Tag (Cat. No. HE2-HA2H5) and anti-CD3 antibody, washed and then analyzed with FACS. FITC signal was used to evaluate the expression of CD3+ T cells in PBMCs, and Alexa Fluor 647 signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).

Protocol

 

背景(Background)

Human Epidermal growth factor Receptor 2 (HER2) is also called ERBB2, HER-2,HER-2 /neu, NEU, NGL,TKR1 and c-erb B2,and is a protein giving higher aggressiveness in breast cancers. It is a member of the ErbB protein family, more commonly known as the epidermal growth factor receptor family. HER2 is a cell membrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. HER2 is thought to be an orphan receptor, with none of the EGF family of ligands able to activate it. Approximately 30% of breast cancers have an amplification of the HER2 gene or overexpression of its protein product. Overexpression of this receptor in breast cancer is associated with increased disease recurrence and worse prognosis. HER2 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation .

 

前沿进展

 
 
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Her2靶点信息
英文全称:Receptor protein-tyrosine kinase erbB-2
中文全称:受体蛋白酪氨酸激酶 erbB-2
种类:Homo sapiens
上市药物数量:35详情
临床药物数量:200详情
最高研发阶段:批准上市
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