APC-Labeled Human BCMA, His Tag (BCA-HA2H4) is produced via conjugation of APC to Human BCMA, His Tag with a new generation site-specific technology under Star Staining labeling platform. Human BCMA, His Tag is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 54 (Accession # Q02223-1 ).
Predicted N-terminus: Met 1
Request for sequence
This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 20.2 kDa.
Excitation Wavelength: 640 nm
Emission Wavelength: 661 nm
Lyophilized from 0.22 μm filtered solution in PBS, 0.2% BSA, pH7.4 with trehalose as protectant.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Mix the 10 ng /well of Monoclonal Anti-Human BCMA Antibody, Human IgG1 with the Donor PAb Anti Human IgG-Eu cryptateHuman IgG1, incubated with the Acceptor APC-Labeled Human BCMA, His Tag (Cat. No. BCA-HA2H4) at increasing concentration. Detection was performed with the EC50 of 0.3848 μg/mL (Routinely tested).
CAR阳性表达率检测（Evaluation of CAR expression）
FACS Analysis of Anti-BCMA CAR Expression
5e5 of anti-BCMA CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of APC-Labeled Human BCMA, His Tag (Cat. No. BCA-HA2H4) and negative control protein respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). APC signal was used to evaluate the binding activity (QC tested).
FACS Analysis of Non-specific binding to PBMCs
5e5 of PBMCs were stained with APC-Labeled Human BCMA, His Tag (Cat. No. BCA-HA2H4) and anti-CD3 antibody, washed and then analyzed with FACS. FITC signal was used to evaluate the expression of CD3+ T cells in PBMCs, and APC signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).
Tumor necrosis factor receptor superfamily member 17 (TNFRSF17) is also known as B-cell maturation protein (BCMA), CD antigen CD269, which is a member of the TNF-receptor superfamily. TNFRSF17 contains one TNFR-Cys repeat. TNFRSF17 is expressed in mature B-cells, but not in T-cells or monocytes. TNFRSF17 is receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. TNFRSF17 promotes B-cell survival and plays a role in the regulation of humoral immunity. TNFRSF17 can activate NF-kappa-B and JNK.