膜杰作
Star Staining
分子别名（Synonym）
Delta3,delta-like 3 (Drosophila),delta-like protein 3,DLL3,Pudgy,SCDO1,SCDO1delta3
表达区间及表达系统（Source）
Alexa Fluor 647-Labeled Human DLL3 Protein, His Tag (DL3-HA2H3) is produced via conjugation of AF647 to Human DLL3 Protein, His Tag with a new generation site-specific technology under Star Staining labeling platform. Human DLL3 Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Leu 492 (Accession # Q9NYJ7-1).
Predicted N-terminus: Ala 27
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 65.7 kDa.
偶联（Conjugate）
AF647
Excitation Wavelength: 640 nm
Emission Wavelength: 672 nm
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
CAR阳性表达率检测（Evaluation of CAR expression）
FACS Analysis of Anti-DLL3 CAR Expression
5e5 of anti-DLL3 CAR-293 cells were stained with 100 μL of 1 μg/mL of Alexa Fluor 647-Labeled Human DLL3 Protein, His Tag (Cat. No. DL3-HA2H3) and negative control protein respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). Alexa 647 signal was used to evaluate the binding activity (QC tested).
Protocol
FACS Analysis of Non-specific binding to PBMCs
5e5 of PBMCs were stained with Alexa Fluor 647-Labeled Human DLL3 Protein, His Tag (Cat. No. DL3-HA2H3) and anti-CD3 antibody, washed and then analyzed with FACS. FITC signal was used to evaluate the expression of CD3+ T cells in PBMCs, and Alexa 647 signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).
Protocol
背景（Background）
Delta-like protein 3 (DLL3) is a transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands.May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm. DLL3 protein is expressed on the surface of tumor cells but not in normal adult tissues.
