膜杰作
Star Staining
ViruStop
Aneuro
ComboX
ActiveMax
GENPower
ClinMax
Star Ribbon
分子别名（Synonym）
MSLN,Mesothelin,MPF
表达区间及表达系统（Source）
FITC-Labeled Human Mesothelin (296-580), His Tag (MSN-HF2H3) is produced via conjugation of FITC to Human Mesothelin (296-580), His Tag with a new generation site-specific technology under Star Staining labeling platform. Human Mesothelin (296-580), His Tag is expressed from human 293 cells (HEK293). It contains AA Glu 296 - Gly 580 (Accession # AAH09272.1).
Predicted N-terminus: Glu 296
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 48.5 kDa. The protein migrates as 50-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Conjugate
FITC
Excitation source: 488 nm spectral line, argon-ion laser
Excitation Wavelength: 488 nm
Emission Wavelength: 535 nm
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
FITC-Labeled Human Mesothelin (296-580), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
CAR阳性表达率检测（Evaluation of CAR expression ）
FACS Analysis of Anti-Mesothelin CAR Expression
2e5 of 293 CAR cells transfected with anti-MSLN-scFv were stained with 100 μL of 3 μg/mL of FITC-Labeled Human Mesothelin (296-580), His Tag (Cat. No. MSN-HF2H3) and negative control protein respectively (Fig. C and B), and non- transfected 293 cells were used as a control (Fig. A), FITC signal was used to evaluate the binding activity (QC tested).
Protocol
FACS Analysis of Non-specific binding to PBMCs
5e5 of PBMCs were stained with FITC-Labeled Human Mesothelin (296-580), His Tag (Cat. No. MSN-HF2H3) and anti-CD3 antibody, washed and then analyzed with FACS. PE signal was used to evaluate the expression of CD3+ T cells in PBMCs, and FITC signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).
Protocol
背景（Background）
Mesothelin (MSLN) is also known as CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, which belongs to the mesothelin family. Mesothelin / MSLN can be proteolytically cleaved into the following two chains by a furin-like convertase: Megakaryocyte-potentiating factor (MPF) and the cleaved form of mesothelin. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro.
