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 >  Protein>Glypican 3 >GP3-HP2H9

PE-Labeled Human Glypican 3 / GPC3 Protein, His TagStar Staining

分子别名(Synonym)

GPC3,OCI5,Glypican-3,GTR2-2,MXR7,DGSX,SDYS ,SGB,SGBS,SGBS1

表达区间及表达系统(Source)

PE-Labeled Human Glypican 3 Protein, His Tag (GP3-HP2H9) is produced via conjugation of PE to Human Glypican 3 Protein, His Tag with a new generation site-specific technology under Star Staining labeling platform. Human Glypican 3 Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Gln 25 - His 559 (Accession # P51654-1 ).

Predicted N-terminus: Gln 25

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the C-terminus

The protein has a calculated MW of 75.3 kDa.

偶联(Conjugate)

PE

Excitation Wavelength: 488 nm / 561 nm

Emission Wavelength: 575 nm

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, 0.2% BSA, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

CAR阳性表达率检测(Evaluation of CAR expression)

FACS Analysis of Anti-Glypican 3 CAR Expression

Glypican 3 CAR_T

5e5 of anti-GPC3 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Human Glypican 3 Protein, His Tag (Cat. No. GP3-HP2H9) and negative control protein respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). PE signal was used to evaluate the binding activity (QC tested).

Protocol

FACS Analysis of Non-specific binding to PBMCs

Glypican 3 CAR_T

5e5 of PBMCs were stained with PE-Labeled Human Glypican 3 Protein, His Tag (Cat. No. GP3-HP2H9) and anti-CD3 antibody, washed and then analyzed with FACS. FITC signal was used to evaluate the expression of CD3+ T cells in PBMCs, and PE signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).

Protocol

 

背景(Background)

Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.

 

前沿进展

 
 
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Glypican 3靶点信息
英文全称:Glypican-3
中文全称:磷脂酰肌醇聚糖3
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:31详情
最高研发阶段:临床二期
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