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 >  Protein>Mesothelin >MSN-HA2H8

Alexa Fluor™ 555-Labeled Human Mesothelin / MSLN (296-580) Protein, His TagStar Staining

分子别名(Synonym)

MSLN,Mesothelin,MPF

表达区间及表达系统(Source)

Alexa Fluor 555-Labeled Human Mesothelin (296-580), His Tag (MSN-HA2H8) is produced via conjugation of AF555 to Human Mesothelin (296-580), His Tag with a new generation site-specific technology under Star Staining labeling platform. Human Mesothelin (296-580), His Tag is expressed from human 293 cells (HEK293). It contains AA Glu 296 - Gly 580 (Accession # Q13421-3 ).

Predicted N-terminus: Glu 296

Request for sequence

蛋白结构(Molecular Characterization)

Mesothelin Structure

This protein carries a polyhistidine tag at the C-terminus

The protein has a calculated MW of 49.1 kDa.

偶联(Conjugate)

AF555

Excitation Wavelength: 561 nm

Emission Wavelength: 572 nm

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

CAR阳性表达率检测(Evaluation of CAR expression)

FACS Analysis of Anti-Mesothelin CAR Expression

Mesothelin CAR_T

5e5 of anti-MSLN CAR-293 cells were stained with 100 μL of 3 μg/mL of AF555-Labeled Human Mesothelin (296-580), His Tag (Cat. No.MSN-HA2H8) and negative control protein respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). AF555 signal was used to evaluate the binding activity (QC tested).

Protocol

FACS Analysis of Non-specific binding to PBMCs

Mesothelin CAR_T

5e5 of PBMCs were stained with AF555-Labeled Human Mesothelin (296-580), His Tag (Cat. No. MSN-HA2H8) and anti-CD3 antibody, washed and then analyzed with FACS. FITC signal was used to evaluate the expression of CD3+ T cells in PBMCs, and AF555 signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).

Protocol

 

背景(Background)

Mesothelin (MSLN) is also known as CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, which belongs to the mesothelin family. Mesothelin / MSLN can be proteolytically cleaved into the following two chains by a furin-like convertase: Megakaryocyte-potentiating factor (MPF) and the cleaved form of mesothelin. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro.

 

前沿进展

 
 
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Mesothelin靶点信息
英文全称:Mesothelin
中文全称:间皮素
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:33详情
最高研发阶段:临床二期
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