登录 | 注册    关注公众号  
微信公众号
搜索
 >  经MALS验证的蛋白>TSLP >TSP-H82E0

Biotinylated Human TSLP (R127A, R130A) Protein, His,Avitag™ (MALS verified)

热销产品推荐:

利用生物素-链霉亲和素系统的高特异性和高灵敏度的结合特性,可将生物素化蛋白与链霉亲和素相关产品联合应用于各类实验,与非生物素体系相比,检测速度更快、结合能力更强、非特异背景更低。

分子别名(Synonym)

TSLP

表达区间及表达系统(Source)

Biotinylated Human TSLP (R127A, R130A) Protein, His,Avitag (TSP-H82E0) is expressed from human 293 cells (HEK293). It contains AA Tyr 29 - Gln 159 (Accession # Q969D9-1 (R127A, R130A)).

Predicted N-terminus: Tyr 29

Request for sequence

蛋白结构(Molecular Characterization)

TSLP Structure

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)

The protein has a calculated MW of 18.5 kDa. The protein migrates as 25-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 12 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

TSLP SDS-PAGE

Biotinylated Human TSLP (R127A, R130A) Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

TSLP SEC-MALS

The purity of Biotinylated Human TSLP (R127A, R130A) Protein, His,Avitag (Cat. No. TSP-H82E0) is more than 90% and the molecular weight of this protein is around 22-32 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

TSLP ELISA

Immobilized Human TSLP R, Fc Tag (Cat. No. TSR-H525a) at 5 μg/mL (100 μL/well) can bind Biotinylated Human TSLP (R127A, R130A) Protein, His,Avitag (Cat. No. TSP-H82E0) with a linear range of 2-31 ng/mL (QC tested).

Protocol

背景(Background)

Thymic stromal lymphopoietin (TSLP) is an epithelial cell-derived cytokine involved in the pathology of inflammatory skin diseases, and is widely expressed by epithelial cells. Human TSLP cDNA encodes a 159 amino acid (aa) residue precursor protein with a 28 aa signal sequence (4, 5). Human TSLP has been shown to developing nondeletional central tolerance, amplifying epithelium-induced class switching, inducing atopic diseases and maintaining intestinal noninflammatory environment. Among diverse cells responding to Human TSLP, CD11c+ dendritic cells are the most obviously characterized target cells.

 

 
货号/价格
产品推荐
文档
联系电话:
400-682-2521(全国)
010-53681107(北京)
021-31168505(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
TSLP靶点信息
英文全称:Thymic stromal lymphopoietin
中文全称:胸腺基质淋巴细胞生成素
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:11详情
最高研发阶段:批准上市
查看更多信息
前沿进展
点击查看详细

消息提示

请输入您的联系方式,再点击提交!

确定