登录 | 注册    关注公众号  
微信公众号
搜索
 >  Protein>EGF R >EGR-M5224

Mouse EGF R Protein, His Tag (MALS verified)

热销产品推荐:

ACROBiosystems提供针对多种生物标志物靶标的lHC抗体产品,具有:验证充分、质量可靠、灵活授权及免疫组化检测服务支持!

分子别名(Synonym)

EGFR,ERBB,ERBB1,HER1,PIG61,mENA

表达区间及表达系统(Source)

Mouse EGF R, His Tag (EGR-M5224) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 647 (Accession # Q01279-1).

Predicted N-terminus: Leu 25

Request for sequence

蛋白结构(Molecular Characterization)

EGF R Structure

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 71.2 kDa. The protein migrates as 86-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

EGF R SDS-PAGE

Mouse EGF R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

EGF R SEC-MALS

The purity of Mouse EGF R, His Tag (Cat. No. EGR-M5224) is more than 90% and the molecular weight of this protein is around 85-110 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

EGF R ELISA

Immobilized Mouse EGF, Fc Tag (Cat. No. EGF-M5265) at 2 μg/mL (100 μL/well) can bind Mouse EGF R, His Tag (Cat. No. EGR-M5224) with a linear range of 0.02-0.313 μg/mL (QC tested).

Protocol

 

活性(Bioactivity)-SPR

EGF R SPR

Captured Mouse EGF R, His Tag (Cat. No. EGR-M5224) on CM5 Chip via anti-His antibody can bind Mouse EGF, Fc Tag (Cat. No. EGF-M5265) with an affinity constant of 5.44 nM as determined in SPR assay (Biacore T200) (Routinely tested).

Protocol

 

背景(Background)

The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.

 

前沿进展

 
 
货号/价格
文档
联系电话:
+86 400-682-2521(全国)
010-53681107(北京)
021-50850665(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
EGF R靶点信息
英文全称:Epidermal growth factor receptor erbB1
中文全称:表皮生长因子受体erbB1
种类:Homo sapiens
上市药物数量:31详情
临床药物数量:169详情
最高研发阶段:批准上市
查看更多信息
前沿进展
点击查看详细

消息提示

请输入您的联系方式,再点击提交!

确定