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 >  Protein>EGF R >EGR-HF2H5

FITC-Labeled Human EGF R Protein, His Tag DMF

分子别名(Synonym)

EGFR,ERBB,ERBB1,HER1,PIG61,mENA

表达区间及表达系统(Source)

FITC-Labeled Human EGF R, His Tag (EGR-HF2H5) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645 (Accession # P00533-1 ). It is the FITC labeled form of Human EGF R, His Tag (EGR-H5222).

Predicted N-terminus: Leu 25

Request for sequence

蛋白结构(Molecular Characterization)

EGF R Structure

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 70.5 kDa. The protein migrates as 85-105 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

偶联(Conjugate)

FITC

Excitation source: 488 nm spectral line, argon-ion laser

Excitation Wavelength: 488 nm

Emission Wavelength: 535 nm

标记(Labeling)

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.

蛋白标记度(Protein Ratio)

The FITC to protein molar ratio is 2-3.5.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

EGF R SDS-PAGE

FITC-Labeled Human EGF R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

EGF R ELISA

Immobilized Cetuximab at 2 μg/mL (100 μL/well) can bind FITC-Labeled Human EGF R, His Tag (Cat. No. EGR-HF2H5) with a linear range of 0.039-0.313 μg/mL (QC tested).

Protocol

 

活性(Bioactivity)-FACS

EGF R FACS

2e5 of Anti-EGFR CAR-293 cells were stained with 100 μL of 1 μg/mL of FITC-Labeled Human EGF R Protein, His Tag (Cat. No. EGR-HF2H5) and negative control protein respectively, FITC signal was used to evaluate the binding activity (QC tested).

Protocol

 

背景(Background)

The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.

文献引用(Citations)

 

前沿进展

 
 
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EGF R靶点信息
英文全称:Epidermal growth factor receptor erbB1
中文全称:表皮生长因子受体erbB1
种类:Homo sapiens
上市药物数量:32详情
临床药物数量:166详情
最高研发阶段:批准上市
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