分子别名(Synonym)
Spike,S protein,Spike glycoprotein,S glycoprotein
表达区间及表达系统(Source)
SARS-CoV-2 Spike Trimer, His Tag (BA.3/Omicron) (SPN-C522c) is expressed from human 293 cells (HEK293). The spike mutations are identified on the SARS-CoV-2 Omicron variant (Pango lineage: BA.3; GISAID clade: GRA; Nextstrain clade: 21M). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.
蛋白结构(Molecular Characterization)
This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 137.8 kDa. The protein migrates as 160-200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂(Formulation)
Supplied as 0.2 μm filtered solution in PBS.
Contact us for customized product form or formulation.
运输(Shipping)
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储(Storage)
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
电泳(SDS-PAGE)
SARS-CoV-2 Spike Trimer, His Tag (BA.3/Omicron) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of SARS-CoV-2 Spike Trimer, His Tag (BA.3/Omicron) (Cat. No. SPN-C522c) is more than 90% and the molecular weight of this protein is around 565-615 kDa verified by SEC-MALS.
Report
活性(Bioactivity)-ELISA
Immobilized SARS-CoV-2 Spike Trimer, His Tag (BA.3/Omicron) (Cat. No. SPN-C522c) at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 2-31 ng/mL (QC tested).
Protocol
Immobilized SARS-CoV-2 Spike Trimer, His Tag (BA.3/Omicron) (Cat. No. SPN-C522c) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (Cat. No. S1N-M122) with a linear range of 0.001-0.013 μg/mL (Routinely tested).
Protocol
背景(Background)
It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.