膜杰作
Star Staining
产品描述（Product Details）
Human iPSC-Derived Intestine Organoids are differentiated from hESC or iPSCs using Human iPSC-Derived Intestine Organoid Differentiation Kit (Ca. No. RIPO-IWM005K). Intestine organoids are three-dimensional in vitro models with a cellular composition and structural organization that is representative to the human intestine regions. The Ready-to-use intestine organoids are derived form human iPS cells and being cultured for more than 60 days. Organoids generated using Human iPSC-Derived intestine Organoid feature various types of cells, including intestine epithelium cells, mesenchyme cells, enterocytes, Paneth cells, goblet cells, etc. These intestine organoids show intestine crypt like structure, Villi and Microvilli like structure. These intestine organoids show regular peristalsis and normal intestinal function validated by the absorption of fatty acid and glucose.
产品特征（Product Specification）
The live organoids are ready-to-use organoids that are delivered in shipping medium and has to go through a 48h recovery process according to instruction.
存储（Storage）
After recovery, please store the organoid in its maintenance medium under the correct incubation condition and medium changing process.
产品示意（Product Diagram）
Protocol Diagram of intestine organoid differentiation.
验证数据（Validation Data）
Organoid Histology and State
The intestine organoids differentiated using the Human iPSC-Derived intestine Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show regular peristalsis.
Orgnaoid Histology
Observation of granulocyte like cells, adipocyte like cells, smooth muscle like cells and intestinal crypt like structure by morphology on day 120 intestine organoids.
Marker Expression
The intestine organoids differentiated using the Human iPSC-Derived intestine Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show expression of goblet cells (mucus-producing, MUC2), brush borders (Villin) and endothelial cells (CD31).
The intestine organoids differentiated using the Human iPSC-Derived intestine Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show expression of enterochromaffin cells (CHGA) and endothelial cells (CD31).
The intestine organoids differentiated using the Human iPSC-Derived intestine Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show expression of goblet cells (mucus-producing, MUC2) and endothelial cells (CD31).
The intestine organoids differentiated using the Human iPSC-Derived intestine Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show expression of smooth muscle cell (α-SMA); epithelial cell (EPCAM); macrophage (CD68); goblet cell (MUC2); Ki67 (intestinal stem cell) and enteroendocrine cells (CHGA).
Organoid Activity
Intestine organoids differentiated using the Human iPSC-Derived intestine Organoid Differentiation Kit (Ca. No. RIPO-IWM005K) show normal intestinal function validated by the absorption of fatty acid and glucose.
