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 >  Protein>NKG2D >NKD-HF224

FITC-Labeled Human NKG2D / CD314 Protein, Fc Tag

分子别名(Synonym)

NKG2D,CD314,KLRK1,NK cell receptor D

表达区间及表达系统(Source)

FITC-Labeled Human NKG2D, Fc Tag (NKD-HF224) is expressed from human 293 cells (HEK293). It contains AA Ile 73 - Val 216 (Accession # P26718-1).

Predicted N-terminus: Pro

Request for sequence

蛋白结构(Molecular Characterization)

NKG2D Structure

This protein carries a human IgG1 Fc tag at the N-terminus

The protein has a calculated MW of 43.1 kDa. The protein migrates as 75-110 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

偶联(Conjugate)

FITC

Excitation source: 488 nm spectral line, argon-ion laser

Excitation Wavelength: 488 nm

Emission Wavelength: 535 nm

标记(Labeling)

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.

蛋白标记度(Protein Ratio)

The FITC to protein molar ratio is 1.5-3.5.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

NKG2D SDS-PAGE

FITC-Labeled Human NKG2D, Fc Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

NKG2D ELISA

Immobilized Human MICA, Fc Tag (MALS verified) (Cat. No. MIA-H5253) at 5 μg/mL (100 μL/well) can bind FITC-Labeled Human NKG2D, Fc Tag (Cat. No. NKD-HF224) with a linear range of 0.156-10 μg/mL (QC tested).

Protocol

 

背景(Background)

NKG2D is a transmembrane protein belonging to the CD94/NKG2 family of C-type lectin-like receptors, also known as KLRK1, CD314, D12S2489E, KLR and killer cell lectin like receptor K1. NKG2D itself forms a homodimer whose ectodomains serve for ligand binding. NKG2D is a major recognition receptor for the detection and elimination of transformed and infected cells as its ligands are induced during cellular stress, either as a result of infection or genomic stress such as in cancer. In NK cells, NKG2D serves as an activating receptor, which itself is able to trigger cytotoxicity. The function of NKG2D on CD8+ T cells is to send co-stimulatory signals to activate them.

 

前沿进展

 
 
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NKG2D靶点信息
英文全称:NKG2-D type II integral membrane protein
中文全称:
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:5详情
最高研发阶段:临床二期
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