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Human CD19-coupled Magnetic Beads

ItemsSize (2mg)Size(5mg X 2)
Particle size2 μm2 μm
Physical appearancePowder mixturePowder mixture
Amount of Coupled Protein>1484 pmol (50 μg) CD19/mg Beads>1484 pmol (50 μg) CD19/mg Beads
Binding Capacity>150 pmol (25 μg) antibody/mg beads>150 pmol (25 μg) antibody/mg beads
FormulationPBS, pH7.4, with 10% TrehalosePBS, pH7.4, with 10% Trehalose
Reconstitution2 mL ultrapure water (1 mg beads/mL)5 mL ultrapure water (1 mg beads/mL)

Background/背景

The biotinylated CD19 protein was conjugated to streptavidin magnetic beads. This pre-coupled magnetic bead product can capture the anti-CD19 antibody from various assay systems. The beads are in uniform size, narrow size distribution with large surface area and unique surface coating, which can help you get the best performance and highly reproducible results. This CD19 coupled magnetic beads will bring great convenience with minimum non-specific binding and developed protocols. This ready-to-use product could greatly save your time and hassle.

Application/应用

This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.

Reconstitution/重构方法

See Certificate of Analysis (CoA) for detailed instruction.

Storage/存储

Upon receipt, please store the Beads at -20℃. The shelf life is 1 year at -20 ℃.

Please avoid more than 3 freeze-thaw cycles. Immediate use after reconstitution is highly recommended.

Assay Principles/原理

The magnetic beads technology makes use of the easy and efficient collection of beads in magnetic field to facilitate antibody purification in a simple workflow of “bind-wash-elute”. In contrast to common separation techniques, this method does not require columns or centrifugation, and is therefore ideal in high-throughput applications.

Antibody Purification:

1. Resuspend the lyophilized beads by adding the buffer of choice.

2. Add analyte to the suspension, mix and incubate to enable specific binding of the beads and the target protein.

3. Magnetize beads, remove supernatant, and wash unbound protein fractions to capture target protein-bound beads.

4. Wash, magnetize the beads and collect purified target protein for use in downstream applications.

 

Typical Data/典型数据

Biotinylated Human ELISA

Immobilized 54 μg CD19 protein to 1 mg Beads, can bind the Anti-CD19 Antibody with an EC50 of 0.8888 μg/mL (QC tested).

Protocol

前沿进展

 
 
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010-57737274(北京)
021-26136428(上海)
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CD19靶点信息
英文全称:B-lymphocyte antigen CD19
中文全称:B淋巴细胞抗原CD19
种类:Homo sapiens
上市药物数量:6详情
临床药物数量:159详情
最高研发阶段:临床三期
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