抗体来源(Source)
Mouse IgG1 Kappa Isotype Control (mAb) is a chimeric monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which combines the variable region of a mouse monoclonal antibody with Mouse IgG1 constant domain. The mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with DNP.
种属(Species)
Mouse
亚型(Isotype)
Mouse IgG1/kappa
抗体类型(Antibody Type)
Recombinant Monoclonal
特异性(Specificity)
Specifically reacts with DNP (Dinitrophenyl) and DNP conjugated proteins.
纯度(Purity)
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
纯化(Purification)
Protein A purified/ Protein G purified
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
25 mg or larger size will be supplied as liquid and shipped by dry ice. Please inquire the dry ice shipping cost.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳(SDS-PAGE)
Mouse IgG1 Kappa Isotype Control (mAb) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Mouse IgG1 Kappa Isotype Control (mAb) (Cat. No. DNP-M1) is more than 90% and the molecular weight of this protein is around 130-160 kDa verified by SEC-MALS.
Report
活性(Bioactivity)-ELISA
Immobilized DNP-Albumin Conjugate, Bovine at 10 μg/mL (100 μL/well) can bind Mouse IgG1 Kappa Isotype Control (mAb) (Cat. No. DNP-M1) with a linear range of 2-20 ng/mL (QC tested).
Protocol
背景(Background)
A hapten is a small molecule that can elicit an immune response only when conjugated with a large carrier such as a protein. Typical haptens include drugs, urushiol, quinone, steroids, etc. Peptides and non-protein antigens usually need conjugating to a carrier protein (such as BSA (bovine serum albumin) or KLH (keyhole limpet hemocyanin) to become good immunogens). Additionally, haptens should be administered with an adjuvant to ensure a high quality immune response. It is important that the hapten design (preserving greatly the chemical structure and spatial conformation of target compound), selection of the appropriate carrier protein and the conjugation method are key conditions for the desired specificity anti-hapten antibodies. We design anti-hapten antibodies based on the HaptenDB information.