表达区间及表达系统（Source）
Dinitrophenyl (DNP) is a hapten that is not normally expressed in the target tissue. Therefore, anti-DNP antibody has no relevant specificity to a target antigen, and can be used as isotype control antibody to differentiate non-specific background signal from specific antibody signal in various in vitro and in vivo studies. Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control was purified from HEK293 cell culture.
亚型（Isotype）
Mouse IgG1/kappa
特异性（Specificity）
Specifically reacts with DNP (Dinitrophenyl) and DNP conjugated proteins.
应用说明（Application）
This antibody is suitable for use as a non-targeting isotype control in various in vitro and in vivo studies. It can also be used as a negative control in various applications such as ELISA, Western blot, immunofluorescence, immunohistochemistry, immunoprecipitation, and flow cytometry. Each laboratory should determine an optimum working titer for use in its particular application.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
内毒素（Endotoxin）
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
存储（Storage）
Avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control(Cat. No. DNP-M1) is more than 90% and the molecular weight of this protein is around 130-160 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized DNP-Albumin Conjugate, Bovine at 10 μg/mL (100 μL/well) can bind Monoclonal Anti-DNP antibody, Mouse IgG1 Isotype Control (Cat. No. DNP-M1) with a linear range of 2-20 ng/mL (QC tested).
Protocol
背景（Background）
A hapten is a small molecule that can elicit an immune response only when conjugated with a large carrier such as a protein. Typical haptens include drugs, urushiol, quinone, steroids, etc. Peptides and non-protein antigens usually need conjugating to a carrier protein (such as BSA (bovine serum albumin) or KLH (keyhole limpet hemocyanin) to become good immunogens). Additionally, haptens should be administered with an adjuvant to ensure a high quality immune response. It is important that the hapten design (preserving greatly the chemical structure and spatial conformation of target compound), selection of the appropriate carrier protein and the conjugation method are key conditions for the desired specificity anti-hapten antibodies. We design anti-hapten antibodies based on the HaptenDB information.
