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Human CD39 / ENTPD1 (Q96A, N99A, E143A, R147E) Protein, His Tag (active enzyme, MALS verified)

分子别名(Synonym)

CD39,ENTPD1,NTPDase 1,Entpd1,Ecto-ATPDase 1,Ecto-ATPase 1

表达区间及表达系统(Source)

Human CD39 (Q96A, N99A, E143A, R147E) Protein, His Tag (CD9-H52H5) is expressed from human 293 cells (HEK293). It contains AA Thr 38 - Val 478 (Accession # P49961-1 (Q96A, N99A, E143A, R147E)).

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蛋白结构(Molecular Characterization)

CD39 Structure

This protein carries a polyhistidine tag at the C-terminus

The protein has a calculated MW of 52.1 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Supplied as 0.2 μm filtered solution in 20 mM Tris,150 mM NaCl,pH8.0 with trehalose as protectant.

Contact us for customized product form or formulation.

运输(Shipping)

This product is supplied and shipped with dry ice, please inquire the shipping cost.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

CD39 SDS-PAGE

Human CD39 (Q96A, N99A, E143A, R147E) Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS

CD39 SEC-MALS

The purity of Human CD39 (Q96A, N99A, E143A, R147E) Protein, His Tag (Cat. No. CD9-H52H5) is more than 90% and the molecular weight of this protein is around 65-85 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

CD39 ELISA

Immobilized Human CD39 / ENTPD1 (Q96A, N99A, E143A, R147E), His Tag (Cat. No. CD9-H52H5) at 1 μg/mL (100 μL/well) can bind X-IgG1 with a linear range of 0.5-3 ng/mL (Routinely tested).

Protocol

 

活性(Bioactivity)

Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-triphosphate (ATP). The specific activity is > 8,000 pmol/min/μg (QC tested).

Protocol

 

背景(Background)

CD39 is also known as Ectonucleoside triphosphate diphosphohydrolase 1, ENTPD1, NTPDase 1, Ecto-ATPDase 1, in the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation by hydrolyzing platelet-activating ADP to AMP. Hydrolyzes ATP and ADP equally well. NTPDase-1 was originally described as CD39, a B lymphocyte cell surface marker, but it is also present on the surface of natural killer cells, T cells, and some endothelial cells.Regulatory T cells(Tregs) mediate immunosuppression through multiple, non-redundant, cell-contact dependent and independent mechanisms, a growing body of evidence suggests an important role for the CD39-CD73-adenosine pathway. CD39 ectonucleotidase is the rate-limiting enzyme of a cascade leading to the generation of suppressive adenosine that alters CD4 and CD8 T cell and natural killer cell antitumor activities.

 

前沿进展

 
 
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CD39靶点信息
英文全称:Ectonucleoside triphosphate diphosphohydrolase 1
中文全称:外核苷三磷酸二磷酸水解酶-1
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:9详情
最高研发阶段:临床二期
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