膜杰作
Star Staining
产品描述（Product Details）
Laminin 411 is a key component of the basement membrane and is involved in the regulation of cell differentiation. Laminin 411 fragment consists of the C-terminal end of the alpha 4, beta 1, and gamma 1 chains.
产品参数（Key parameter）
| Purity (SDS PAGE) | > 90% |
| Endotoxin Test | < 1.0 EU per μg |
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
活性（Bioactivity）-ELISA
Immobilized Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W7) at 5 μg/mL (100 μL/well) can bind Human Laminin 411 Protein (Cat. No. LAT-H5263) with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
Immobilized Human ITGA7X2B&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W8) at 5 μg/mL (100 μL/well) can bind Human Laminin 411 Protein (Cat. No. LAT-H5263) with a linear range of 0.01-0.625 μg/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Human Laminin 411 Protein (Cat. No. LAT-H5263) immobilized on CM5 Chip can bind Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W7) with an affinity constant of 29.3 nM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
Human Laminin 411 Protein (Cat. No. LAT-H5263) immobilized on CM5 Chip can bind Human ITGA7X2B&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W8) with an affinity constant of 17.4 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Human MCAM, His Tag (Cat. No. CD6-H5229) immobilized on CM5 Chip can bind Human Laminin 411 Protein (Cat. No. LAT-H5263) with an affinity constant of 0.115 µM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Human MCAM, His Tag (Cat. No. CD6-H5229) captured on CM5 chip via anti-His antibody can bind Human Laminin 411 Protein (Cat. No. LAT-H5263) with an affinity constant of 27.9 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Human Laminin 411 Protein (Cat. No. LAT-H5263) captured on Protein A Chip can bind Human MCAM, His Tag (Cat. No. CD6-H5229) with an affinity constant of 107 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Human Laminin 411 Protein (Cat. No. LAT-H5263) on Protein A Biosensor, can bind Human ITGA6&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W7) with an affinity constant of 26.9 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Protocol
