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Human Leptin R / CD295 Protein, Fc Tag

分子别名(Synonym)

LEPR,CD295,DKFZp686B1731,OBR,Leptin receptor

表达区间及表达系统(Source)

Human Leptin R, Fc Tag (LER-H5252) is expressed from human 293 cells (HEK293). It contains AA Phe 22 - Asp 839 (Accession # NP_002294.2).

Predicted N-terminus: Phe 22

蛋白结构(Molecular Characterization)

Online(Phe 22 - Asp 839) NP_002294.2

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 119.6 kDa. The protein migrates as 150-165 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Human Leptin R, Fc Tag (Cat. No. LER-H5252) SDS-PAGE gel

Human Leptin R, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

背景(Background)

Leptin receptor (LEPR) is also known as LEP-R, cluster of differentiation 295 (CD295), OB-R and B219, is a single-transmembrane-domain receptor of the gp130 family of cytokine receptors. Leptin receptor exists as homodimer and binds Leptin with high affinity, thus mediates the biological function of the adipocyte-specific hormone Leptin. LEPR is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this protein have been associated with obesity and pituitary dysfunction. Interaction of leptin and leptin receptor is crucial for body weight and bone mass regulation in mammals through hypothalamic effects on satiety and energy expenditure. Meanwhile, research data supports a leptin receptor activation model based on ligand-induced conformational changes.

 

前沿进展

 
货号/价格

LER-H5252-100ug / ¥2900

LER-H5252-250ug / ¥5800

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Leptin R靶点信息
英文全称:Leptin receptor
中文全称:瘦素受体
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:2详情
最高研发阶段:批准上市
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