膜杰作
Star Staining
描述（Description）
The Human TSHR (Luc) HEK293 Reporter Cell was engineered to not only express CREB signaling response element, but also express the receptor full length human TSHR (Gene ID: 7253). When stimulated with human TSH protein or TSHR agonist antibody, receptor-mediated signaling can drive CREB-mediated luminescence. Neutralization of biological effect of human TSH protein by corresponding antibody results in a decrease in luminescence.
应用说明（Application）
• Screen for anti-human TSHR agonist antibody.
• Screen for neutralizing antibodies blocking the stimulation of human TSH protein.
生长特性（Growth Properties）
Adherent
筛选标记（Selection Marker）
Puromycin (2 μg/mL) + Hygromycin (20 μg/mL)
培养基（Culture Medium）
DMEM medium + 10% FBS
冻存液（Freeze Medium）
Serum-free cell cryopreservation medium
装量（Quantity）
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
存储（Storage）
Frozen in liquid nitrogen.
支原体检测（Mycoplasma Testing）
Negative
无菌检测（Sterility Testing）
Negative
使用说明（Instructions for Use）
See data sheet for detailed culturing and assay protocol.
Receptor Assay
Expression analysis of human TSHR on Human TSHR (Luc) HEK293 Reporter Cell by FACS.
Cell surface staining was performed on Human TSHR (Luc) HEK293 Reporter Cell or negative control cell using anti-human TSHR agonistic antibody followed by staining with PE anti-human IgG Fc antibody.
Protocol
Application
Agonistic activity analysis of anti-human TSHR antibody.
The Human TSHR (Luc) HEK293 Reporter Cell was incubated with serial dilutions of anti-human TSHR agonistic antibody. The EC50 of anti-human TSHR agonistic antibody was approximately 0.1767 μg/mL with the max induction fold 30.53.
Protocol
Inhibition of human TSH protein-induced reporter activity by anti-human TSHR neutralizing antibody.
The Human TSHR (Luc) HEK293 Reporter Cell was incubated with serial dilutions of antibodies in the presence of human TSH alpha/beta Heterodimer protein (Cat.No.TSR-H52W8) with a final concentration of 0.2 μg/mL. The EC50 of anti-human TSHR neutralizing antibody is approximately 1.167 μg/mL.
Protocol
Signaling Bioassay
Response to human TSH protein (RLU).
The Human TSHR (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human TSH alpha/beta Heterodimer protein (Cat.No.TSR-H52W8). The EC50 was approximately 0.4685 μg/mL.
Protocol
Response to human TSH protein (FOLD).
The Human TSHR (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human TSH alpha/beta Heterodimer protein (Cat.No.TSR-H52W8). The max induction fold was approximately 24.38.
Protocol
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背景（Background）
The protein encoded by this gene is a membrane protein and a major controller of thyroid cell metabolism. The encoded protein is a receptor for thyrothropin and thyrostimulin, and its activity is mediated by adenylate cyclase. Defects in this gene are a cause of several types of hyperthyroidism. Three transcript variants encoding different isoforms have been found for this gene.
License Disclosure
This cell line is provided for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit modification of this cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license. Modifications of this cell line, transfer to another facility, or commercial use of the cell lines may require a separate license and additional fees. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.
