膜杰作
Star Staining
表达区间及表达系统（Source）
APC-Labeled Human HLA-E*01:03&B2M&EBV BZLF-1 (SQAPLPCVL) Tetramer Protein (HLF-HA2H7) is expressed from human 293 cells (HEK293). It contains AA Gly 22 - Ile 305 (HLA-E*01:03) & Ile 21 - Met 119 (B2M) & SQAPLPCVL peptide (Accession # P13747 (HLA-E*01:03) & P61769-1 (B2M) & SQAPLPCVL).
Predicted N-terminus: Gly 22 & Ile 21
Request for sequence
蛋白结构（Molecular Characterization）
APC-Labeled Human HLA-E*01:03&B2M&EBV BZLF-1 (SQAPLPCVL) Tetramer Protein is assembled by biotinylated monomer and APC-labeled streptavidin.
Biotinylated Human HLA-E*01:03&B2M&EBV BZLF-1 (SQAPLPCVL) Complex Protein is produced by co-expression of HLA and B2M loaded with EBV BZLF-1 peptide. Biotinylated Human HLA-E*01:03&B2M&EBV BZLF-1 (SQAPLPCVL) Complex Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
偶联（Conjugate）
APC
Excitation Wavelength: 640 nm
Emission Wavelength: 661 nm
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, 1% BSA, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
背景（Background）
HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail.
