膜杰作
Star Staining
分子别名（Synonym）
IFNAR1,IFNAR,CRF2-1,IFN-R-1,IFNAR-1
表达区间及表达系统（Source）
Cynomolgus IFN-alpha/beta R1 Protein, His Tag (IF1-C52H3) is expressed from human 293 cells (HEK293). It contains AA Ala 25 - Lys 437 (Accession # A0A7N9D7J0-1).
Predicted N-terminus: Ala 25
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 49.3 kDa. The protein migrates as 65-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Cynomolgus IFN-alpha/beta R1 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Cynomolgus IFN-alpha/beta R1 Protein, His Tag (Cat. No. IF1-C52H3) is more than 85% and the molecular weight of this protein is around 65-85 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human IFN-alpha 1, Fc Tag (Cat. No. IFA-H5258) at 5 μg/mL (100 μL/well) can bind Cynomolgus IFN-alpha/beta R1 Protein, His Tag (Cat. No. IF1-C52H3) with a linear range of 5-78 ng/mL (QC tested).
Protocol
活性（Bioactivity）-BLI
Loaded bind Human IFN-alpha 1, Fc Tag (Cat. No. IFA-H5258) on Protein A Biosensor, can bind Cynomolgus IFN-alpha/beta R1 Protein, His Tag (Cat. No. IF1-C52H3) with an affinity constant of 16.5 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
背景（Background）
Interferon alpha/beta receptor 1 ( IFNAR1) is also known as Cytokine receptor class-II member 1, Cytokine receptor family 2 member 1 (CRF2-1), Type I interferon receptor 1, IFNAR, which belongs to the type II cytokine receptor family. IFNAR1 /IFNAR contains four fibronectin type-III domains. IFNAR1 associates with IFNAR2 to form the type I interferon receptor. IFNAR1 is receptor for interferons alpha and beta. IFNAR1 can transduce IFNB signals without the help of IFNAR2, and not activating the Jak-STAT pathway.
