膜杰作
Star Staining
表达区间及表达系统（Source）
APC-Labeled Mouse H-2Db&B2M&TRP1 (TAPDNLGYA) Tetramer Protein (H2P-HA2H7) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Val 309 (H-2Db) & Ile 21 - Met 119 (B2M) & TAPDNLGYA peptide (Accession # P01899 (H-2Db) & P01887 (B2M) & TAPDNLGYA).
Predicted N-terminus: Gly 25 & Ile 21
蛋白结构（Molecular Characterization）
APC-Labeled Mouse H-2Db&B2M&TRP1 (TAPDNLGYA) Tetramer Protein is assembled by biotinylated monomer and APC-labeled streptavidin.
Biotinylated Mouse H-2Db&B2M&TRP1 (TAPDNLGYA) Complex Protein is produced by co-expression of H-2Db and B2M loaded with TRP1 peptide. Biotinylated Mouse H-2Db&B2M&TRP1 (TAPDNLGYA) Complex Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
偶联（Conjugate）
APC
Excitation Wavelength: 640 nm
Emission Wavelength: 661 nm
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, 1% BSA, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
活性（Bioactivity）-ELISA
Immobilized APC-Labeled Mouse H-2Db&B2M&TRP1 (TAPDNLGYA) Tetramer Protein (Cat. No. H2P-HA2H7) at 1 μg/mL (100 μL/well) can bind H-2Kb/H2D Monoclonal Antibody (5041.16.1) Biotin with a linear range of 1-16 ng/mL (Routinely tested).
Protocol
背景（Background）
TRP1 refers to tyrosinase-related protein. TRP1 is encoded by the TYRP1 gene. It is the human homologue of the brown (b)-locus in mice, the corresponding protein of which is associated with melanogenesis and pigmentation disorders of the skin. The exact function of TRP1 is still unknown. Besides its enzymatic activity, it may also play a structural role in the melanosome. TYRP1 is involved in the production of eumelanin, but it has also been implicated in the maintenance of the ultrastructure of melanosomes, melanocyte proliferation, and apoptosis. Defects in TYRP1 are the cause of albinism oculocutaneous type 3 (OCA3).
