膜杰作
Star Staining
表达区间及表达系统（Source）
Biotinylated Human IL-7 R alpha & IL-2 R gamma Protein, His,Avitag&Tag Free (ILA-H82W3) is expressed from human 293 cells (HEK293). It contains AA Glu 21 - Asp 239 & Leu 23 - Asn 254 (Accession # P16871-1 & P31785-1).
Predicted N-terminus: Glu 21 & Leu 23
蛋白结构（Molecular Characterization）
Biotinylated Human IL-7 R alpha & IL-2 R gamma Protein has a calculated MW of 32.2 kDa (IL-7 R alpha) & 31.2 kDa (IL-2 R gamma). The protein migrates as 43-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Biotinylated Human IL-7 R alpha & IL-2 R gamma Protein, His,Avitag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human IL-7 R alpha & IL-2 R gamma Protein, His,Avitag&Tag Free (Cat. No. ILA-H82W3) is more than 90% and the molecular weight of this protein is around 95-115 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human IL-7, Fc Tag (Cat. No. IL7-H5253) at 2 μg/mL (100 μL/well) can bind Biotinylated Human IL-7 R alpha & IL-2 R gamma Protein, His,Avitag&Tag Free (Cat. No. ILA-H82W3) with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
背景（Background）
The protein encoded by this gene is a receptor for interleukin 7 (IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukins 2, 4, 7, 9, and 15. This protein has been shown to play a critical role in V(D)J recombination during lymphocyte development. Defects in this gene may be associated with severe combined immunodeficiency (SCID). Alternatively spliced transcript variants have been found.
