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 >  Protein>CCR5 >CC5-H52P5

Fluorescent Human CCR5 Full Length Protein (VLP)

分子别名(Synonym)

CCR5,CMKBR5,CD195,CC-CKR-5,CHEMR13

表达区间及表达系统(Source)

Fluorescent Human CCR5 Full Length Protein (VLP) (CC5-H52P5) is expressed from human 293 cells (HEK293).

蛋白结构(Molecular Characterization)

This protein carries a GFP tag.

The protein has a calculated MW of 40.5 kDa | 83.1 kDa.

Virus-like particles(VLPs) are formed by self-assembly of envelop/capsid proteins from viruses. Membrane Proteins can be constituted in-situ with VLPs produced from HEK293 cell cultures. These VLPs concentrate conformationally intact membrane proteins directly on the cell surface and produce soluble, high-concentration proteins perfect for immunization and antibody screening.

The VLPs provide the display of properly folded membrane proteins in their native cellular membrane in a compact size of 100~300 nm diameter (similar to the size of most viruses) making it optimal targets for dendritic cells in vivo and surface attachment for phage display.

偶联(Conjugate)

GFP

Excitation source: 488 nm spectral line, argon-ion laser

Excitation Wavelength: 488 nm

Emission Wavelength: 530 nm

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

制剂(Formulation)

The VLPs are highly immunogenic, so the immunization strategy should be optimized (antigen dose, regimen and adjuvant).

Supplied as 0.2 μm filtered solution in PBS, Arginine, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

运输(Shipping)

This product is supplied and shipped with dry ice, please inquire the shipping cost.

存储(Storage)

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. The product MUST be stored at -70°C or lower upon receipt;
  2. -70°C for 12 months under sterile conditions.
*The isotype control of empty/mock VLP (Cat. No. VLP-NF2P4) is sold separately and not included in protein, you can follow this link for product information.
 

活性(Bioactivity)-ELISA

CCR5 ELISA

Immobilized Fluorescent Human CCR5 Full Length Protein (VLP) (Cat. No. CC5-H52P5) at 2 μg/mL (100 μL/well) can bind Anti-Human CD195/CCR5 Antibody with a linear range of 2-78 ng/mL (QC tested).

Protocol

 

均一性(Identity)-DLS

CCR5 DLS

The mean peak Radius of VLP is 60-80 nm with more than 95% intensity as determined by dynamic light scattering (DLS).

 

背景(Background)

Receptor for a number of inflammatory CC-chemokines including CCL3/MIP-1-alpha, CCL4/MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation.5 Publications (Microbial infection) Acts as a coreceptor (CD4 being the primary receptor) of human immunodeficiency virus-1/HIV-1.

 

前沿进展

 
 
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CCR5靶点信息
英文全称:C-C chemokine receptor type 5
中文全称:C-C趋化因子受体5型
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:13详情
最高研发阶段:批准上市
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