登录 | 注册    关注公众号  
微信公众号
搜索
 >  其他蛋白>VLP >VLP-NF2P4

Fluorescent Virus-Like Particle (VLP) isotype control

分子别名(Synonym)

VLP,Virus Like Particle,eVLP,VLPs

表达区间及表达系统(Source)

Fluorescent VLP isotype control (VLP-NF2P4) is expressed from human 293 cells (HEK293).

蛋白结构(Molecular Characterization)

Fluorescent VLP isotype control (VLP-NF2P4) carries a GFP tag and is formed by self-assembly of envelop/capsid proteins from viruses, it can be used as isoytype control for Fluorescent VLPs displaying membrane proteins in various applications.

Virus-like particles(VLPs) are formed by self-assembly of envelop/capsid proteins from viruses. Membrance Proteins can be constituted in-situ with VLPs produced from HEK293 cell cultures. These VLPs concentrate conformationally intact membrane proteins directly on the cell surface and produce soluble, high-concentration proteins perfect for immunization and antibody screening.

The VLPs provide the display of properly folded membrane proteins in their native cellular membrane in a compact size of 100~300 nm diameter (similar to the size of most viruses) making it optimal targets for dendritic cells in vivo and surface attachment for phage display.

偶联(Conjugate)

GFP

Excitation source: 488 nm spectral line, argon-ion laser

Excitation Wavelength: 488 nm

Emission Wavelength: 530 nm

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

The VLPs are highly immunogenic, so the immunization strategy should be optimized (antigen dose, regimen and adjuvant).

Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

运输(Shipping)

This product is supplied and shipped as sterile liquid solution with dry ice, please inquire the shipping cost.

存储(Storage)

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. The product MUST be stored at -70°C or lower upon receipt;
  2. -70°C for 3 months under sterile conditions.
 

均一性(Identity)-DLS

VLP DLS

The mean peak Radius of VLP is 65-85 nm with more than 95% intensity as determined by dynamic light scattering (DLS).

背景(Background)

Virus-like particles (VLPs) are highly structured protein particles composed of single or multiple structural proteins of the virus. Its morphological structure is similar to that of natural virus particles, does not contain virus accounting, and has strong immunogenicity and biological activity. The empty shell structure of most VLP is icosahedral or helical structure, which is composed of several specific structural proteins. At present, it is widely used in vaccine production and antigen presentation.

 

前沿进展

 
 
货号/价格
文档
联系电话:
400-682-2521(全国)
010-53681107(北京)
021-31168505(上海)
运输方式
订单邮箱:
order.cn@acrobiosystems.com
技术支持邮箱:
tech.cn@acrobiosystems.com
前沿进展
点击查看详细
相关产品
View All VLP

消息提示

请输入您的联系方式,再点击提交!

确定