膜杰作
Star Staining
产品描述（Product Details）
| Assay Type | Sandwich-ELISA |
| Analyte | DLL4 |
| Format | 96T |
| Reactivity | Human |
| Regulatory Status | RUO |
| Sensitivity | < 18.75 pg/mL |
| Standard Curve Range | 18.75 pg/mL-1200 pg/mL |
| Assay Time | 3 hr 20 min |
| Suitable Sample Type | For the quantitative determination of human DLL4 in Cell Culture Supernatants, Plasma, Serum. |
| Sample volume | 100 uL |
组分（Materials Provided）
| ID | Components | Size |
| RES052-C01 | Pre-coated Anti-DLL4 Antibody Microplate | 1 plate |
| RES052-C02 | Human Fc Tag DLL4 Standard | 20 μg |
| RES052-C03 | Biotin-Anti-DLL4 Antibody | 150 μL |
| RES052-C04 | Streptavidin-HRP | 50 μL |
| RES052-C05 | 10xWashing Buffer | 50 mL |
| RES052-C06 | 2xDilution Buffer | 50 mL |
| RES052-C07 | Substrate Solution | 12 mL |
| RES052-C08 | Stop Solution | 7 mL |
背景（Background）
Human Fc Tag DLL4 ELISA KIT is based on ELISA sandwich method and designed to measure human Fc Tag DLL4 levels in cell culture supernates, serum, and plasma. It contains recombinant human Fc Tag DLL4 and a pair of antibodies against the recombinant factor, which are provided by ACROBiosystems. Results are obtained by four parameter logistic curve that were parallel to the standard curves obtained. The verification results indicate that this kit can be used for the quantitative determination of GMP human Fc Tag DLL4 (ACROBiosystems, cat#GMP-DL4H28) concentrations. The specificity has been verified.
应用说明（Application）
Human Fc Tag DLL4 ELISA KIT was developed for the detection and quantitative determination of GMP human Fc Tag DLL4(ACROBiosystems, cat#GMP-GMP-DL4H28) concentration.
It is for research use only.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储（Storage）
Unopened kit should be stored at 2°C -8°C upon receiving.
Find the expiration date on the outside packaging and do not use reagents past their expiration date.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
原理（Assay Principles）
This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of Human Fc Tag DLL4. The kit consists of Pre-coated Anti-DLL4 Antibody Microplate and Human Fc Tag DLL4 Standard and Biotin-Anti-DLL4 Antibody and Streptavidin-HRP and buffers.
Your experiment will include 6 simple steps:
a) Bring all reagents to room temperature(20℃-25℃) before use.
b) Add your sample to the plate and take the Human Fc Tag DLL4 Standard. The samples and standard are diluted by Dilution Buffer.
c) Wash the plate and add the Biotin-Anti-DLL4 Antibody diluted by Dilution Buffer to the plate.
d) Wash the plate and add the Streptavidin-HRP diluted by Dilution Buffer to the plate.
e) Wash the plate and add TMB.
f) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated by the absorbance at 450 nm minus the absorbance at 630 nm to remove background disturbance before statistical analysis. The OD Value reflects the amount of bound protein.
典型数据-Typical Data Please refer to Ds document for the assay protocol.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
验证（Validation）
稀释线性（Dilution Linearity）
To assess the linearity of the assay, samples spiked with high concentrations of human Fc Tag DLL4 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
批内差异（Intra-Assay Statistics）
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision, Intra-Assay Precision CV＜10%.
批间差异（Inter-Assay Statistics）
Three samples of known concentration were tested in three separate assays to assess inter-assay precision, Inter-Assay Precision CV<10%.
回收率（Recovery）
Three parts of blank serum were added with different concentrations of human Fc Tag DLL4, and the serum without human Fc Tag DLL4 was used as background to calculate the recovery rate. The range of the recovery rate is 83.7-116.5%, and the average recovery is 98.6%.
背景（Background）：Fc Tag DLL4
Delta-like protein 4 (DLL4) is also known as Drosophila Delta homolog 4 (Delta4), which contains one DSL domain and eight EGF-like domains. DLL4 is expressed in vascular endothelium. DLL4 is involved in the Notch signaling pathway as Notch ligand, which can activates NOTCH1 and NOTCH4. DLL4 is involved in angiogenesis and negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. DLL4 can bind to Notch-1 and Notch-4.
