膜杰作
Star Staining
分子别名（Synonym）
Fusion glycoprotein F0
表达区间及表达系统（Source）
Mumps virus (strain Miyahara vaccine) (MuV) Fusion glycoprotein F0, His Tag (RSF-V52H4) is expressed from human 293 cells (HEK293). It contains AA Val 20 - ILe 486(FAGIAIGIA 103-111del) (Accession # P11236 (FAGIAIGIA 103-111del)).
Predicted N-terminus: Val 20
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 51.9 kDa. The protein migrates as 53-63 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Mumps virus (strain Miyahara vaccine) (MuV) Fusion glycoprotein F0, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Mumps virus (strain Miyahara vaccine) (MuV) Fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H4) is more than 85% and the molecular weight of this protein is around 185-215 kDa verified by SEC-MALS.
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背景（Background）
Mumps immunity is typically assessed by measuring neutralizing-antibody responses directed against mumps HN and F proteins.
