膜杰作
Star Staining
分子别名（Synonym）
CD6,TP120,T12
表达区间及表达系统（Source）
Biotinylated Cynomolgus CD6 Protein, His,Avitag (CD6-C82E3) is expressed from human 293 cells (HEK293). It contains AA His 18 - Leu 399 (Accession # XP_005577746.1).
Predicted N-terminus: His 18
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 44.4 kDa. The protein migrates as 63-90 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Biotinylated Cynomolgus CD6 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Cynomolgus CD6 Protein, His,Avitag (Cat. No. CD6-C82E3) is more than 90% and the molecular weight of this protein is around 70-90 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Biotinylated Cynomolgus CD6 Protein, His,Avitag (Cat. No. CD6-C82E3) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Human ALCAM, Fc Tag (Cat. No. CD6-H5259) with a linear range of 0.3-2 ng/mL (QC tested).
Protocol
Immobilized Human ALCAM, Fc Tag (Cat. No. CD6-H5259) at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus CD6 Protein, His,Avitag (Cat. No. CD6-C82E3) with a linear range of 0.01-0.313 μg/mL (Routinely tested).
Protocol
Immobilized Human ALCAM, His Tag (Cat. No. CD6-H5228) at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus CD6 Protein, His,Avitag (Cat. No. CD6-C82E3) with a linear range of 0.01-0.625 μg/mL (Routinely tested).
Protocol
背景（Background）
CD6 (Cluster of Differentiation 6) is a cell surface glycoprotein that is involved in T cell activation and a member of the scavenger receptor cysteine rich protein superfamily (SRCRSF). CD6 is expressed at low levels on immature thymocytes and at high levels on mature thymocytes. The majority of peripheral blood T cells, a subset of B cells, and a subset of neuronal cells express CD6. CD6 appears to play a role as both a costimulatory molecule in T cell activation and as an adhesion receptor. Studies demonstrating a mitogenic effect for T cells with some CD6 specific monoclonal antibodies, in conjunction with either accessory cells or PMA and anti-CD2 mAb, support the concept of CD6 as a co stimulatory molecule.
