膜杰作
Star Staining
分子别名（Synonym）
CEACAM-5,CD66e,CEA,Meconium antigen 100
表达区间及表达系统（Source）
Human CEACAM-5 (499-685) Protein, His Tag (CE5-H52H8) is expressed from human 293 cells (HEK293). It contains AA Glu 499 - Ala 685 (Accession # P06731-1).
Predicted N-terminus: Glu 499
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 21.9 kDa. The protein migrates as 40-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
SEC-MALS
The purity of Human CEACAM-5 (499-685) Protein, His Tag (Cat. No. CE5-H52H8) is more than 85% and the molecular weight of this protein is around 33-45 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human CEACAM-5 (499-685) Protein, His Tag (Cat. No. CE5-H52H8) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CEACAM5 Antibody, Human IgG1 with a linear range of 0.076-1 ng/mL (QC tested).
Protocol
背景（Background）
Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) is also Carcinoembryonic antigen (CEA), Meconium antigen 100, CD antigen CD66e, CEACAM5 belongs to the immunoglobulin superfamily and CEA family. CEACAM5 contains seven Ig-like (immunoglobulin-like) domains. CEACAM5 is homodimer protein which binding of E.coli Dr adhesins leads to dissociation of the homodimer. CEACAM5 is cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. CEACAM5 is receptor for E.coli Dr adhesins.
