膜杰作
Star Staining
分子别名（Synonym）
OX40L,TNFSF4,CD252,Glycoprotein Gp34,TXGP1,CD134 ligand,CD134L
表达区间及表达系统（Source）
Human OX40 Ligand Protein, His Tag (OXL-H5243) is expressed from human 293 cells (HEK293). It contains AA Gln 51 - Leu 183 (Accession # P23510-1).
Predicted N-terminus: His
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 49.4 kDa. The protein migrates as 50-116 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>80% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human OX40 Ligand Protein, His Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 80%.
SEC-MALS
The purity of Human OX40 Ligand Protein, His Tag (Cat. No. OXL-H5243) is more than 95% and the molecular weight of this protein is around 55-75 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human OX40 Ligand Protein, His Tag (Cat. No. OXL-H5243) at 2 μg/mL (100 μL/well) can bind Human OX40, Fc Tag (Cat. No. OX0-H5255) with a linear range of 0.1-1 ng/mL (QC tested).
Protocol
Immobilized Human OX40 Ligand Protein, His Tag (Cat. No. OXL-H5243) at 2 μg/mL (100 μL/well) can bind Human OX40 Protein, Mouse IgG2a Fc Tag (Cat. No. OX0-H5252) with a linear range of 0.2-8 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Biotinylated Human OX40, Avitag,His Tag (Cat. No. TN4-H82E4) on SA Biosensor, can bind Human OX40 Ligand Protein, His Tag (Cat. No. OXL-H5243) with an affinity constant of 91.3 pM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
背景（Background）
Tumor necrosis factor ligand superfamily member 4 (TNFSF4) is also known as glycoprotein Gp34, OX40 ligand (OX40L), TAX transcriptionally-activated glycoprotein 1 and CD252, which belongs to the tumor necrosis factor family. TNFSF4 is the ligand for CD134 and is expressed on such cells as DC2s (a subtype of dendritic cells) enabling amplification of Th2 cell differentiation. The interaction of TNFSF4-TNFSF4 is involved in the pathogenesis of multiple autoimmune and inflammatory diseases such as systemic lupus erythematosus (SLE), carotid artery disease and cancer. Furthermore, similar to other TNF superfamily members, membrane-bound OX40 Ligand (TNFSF4) exists as a homotrimer. Human TNFSF4 shares 46% amino acid sequence identity with its mouse counterpart.
