膜杰作
Star Staining
描述（Description）
The Human RANK (Luc) HEK293 Reporter Cell was engineered to express NF-kB signaling response element driving luciferase expressing systems. When stimulated with human RANKL protein, the RANK/RANKL interaction drives NF-kB-mediated luminescence. Blocking the RANK/RANKL interaction by either anti-RANK or anti-RANKL antibodies results in a decrease in luminescence.
应用说明（Application）
• Screen for anti-human RANK antibody or anti-human RANKL antibody blocking RANK/RANKL interaction.
生长特性（Growth Properties）
Adherent
筛选标记（Selection Marker）
Puromycin (2 μg/mL)
培养基（Culture Medium）
DMEM medium + 10% FBS
冻存液（Freeze Medium）
Serum-free cell cryopreservation medium
装量（Quantity）
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
存储（Storage）
Frozen in liquid nitrogen.
支原体检测（Mycoplasma Testing）
Negative
无菌检测（Sterility Testing）
Negative
使用说明（Instructions for Use）
See data sheet for detailed culturing and assay protocol.
Application
Inhibition of human RANKL protein-induced reporter activity by anti-human RANKL neutralizing antibody.
This reporter cell was incubated with serial dilutions of antibodies in the presence of human RANKL protein (Cat.No.RAL-H5240) with a final concentration of 0.3 μg/mL. The EC50 of anti-human RANKL neutralizing antibody is approximately 0.1742 μg/mL.
Protocol
Signaling Bioassay
Response to human RANKL protein (RLU).
The Human RANK (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human RANKL protein (Cat.No.RAL-H5240). The EC50 was approximately 0.89 μg/mL.
Protocol
Response to human RANKL protein (FOLD). The Human RANK (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human RANKL protein (Cat.No.RAL-H5240). The max induction fold was approximately 30.13.
Protocol
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背景（Background）
RANK / TNFRSF11A is a member of the tumor necrosis factor receptor family. RANK / TNFRSF11A shares significant amino acid homology with other members of the TNF R family in its extracellular four cysteine-rich repeats. Functional genomics in mice showed that RANKL (TNFSF11), a member of the TNF ligand superfamily, and RANK (TNFRSF11a), the cognate TNFR family receptor for RANKL, were essential for osteoclastogenesis in vivo. A functional interaction between RANKL, expressed by bone stromal cells of the osteoblast lineage, and RANK, expressed by osteoclast precursors of hematopoietic myeloid lineage, is necessary for osteoclast differentiation, survival, and activation.
License Disclosure
This cell line is provided for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit modification of this cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license. Modifications of this cell line, transfer to another facility, or commercial use of the cell lines may require a separate license and additional fees. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.
