膜杰作
Star Staining
分子别名（Synonym）
uPAR,PLAUR,CD87,MO3
表达区间及表达系统（Source）
APC-Labeled Human uPAR Protein, His Tag (UPR-HA2H9) is produced via conjugation of APC to Human uPAR Protein, His Tag with a new generation site-specific technology under optimal conditions with a proprietary technology. Human uPAR Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Arg 303 (Accession # Q03405-1).
Predicted N-terminus: Leu 23
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 35.0 kDa.
偶联（Conjugate）
APC
Excitation Wavelength: 640 nm
Emission Wavelength: 661 nm
应用说明（Application）
Please note that this product is NOT compatible to streptavidin detection system.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
活性（Bioactivity）-FACS
5e5 of anti-uPAR CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of APC-Labeled Human uPAR Protein, His Tag (Cat. No. UPR-HA2H9) and negative control protein respectively. APC signal was used to evaluate the binding activity (QC tested).
Protocol
背景（Background）
Urokinase plasminogen activator surface receptor (U-PAR) is also known as PLAUR, Monocyte activation antigen Mo3, CD antigen CD87. PLAUR contains three UPAR/Ly6 domains. U-PAR is expressed in neurons of the rolandic area of the brain (at protein level) and is also expressed in the brain. PLAUR / CD87 interacts with MRC2, SRPX2 and SORL1. PLAUR / UPAR acts as a receptor for urokinase plasminogen activator and plays a role in localizing and promoting plasmin formation. U-PAR mediates the proteolysis-independent signal transduction activation effects of U-PA.
