膜杰作
Star Staining
描述（Description）
The TCF/LEF (Luc) HEK293 Reporter Cell was engineered to express TCF/LEF signaling response element driving luciferase expressing systems, designed for monitoring the activity of the Wnt/ß-catenin signaling pathway. When stimulated with human Wnt protein, receptor-mediated signaling can drive TCF/LEF-mediated luminescence. Inhibition of biological effect of human Wnt protein by corresponding inhibitors results in a decrease in luminescence.
应用说明（Application）
• Monitor Wnt signaling pathway activity.
• Screen for Wnt/β-catenin targeted agents.
生长特性（Growth Properties）
Adherent
筛选标记（Selection Marker）
Puromycin (2 μg/mL)
培养基（Culture Medium）
DMEM + 10% FBS
冻存液（Freeze Medium）
Serum-free cell cryopreservation medium
装量（Quantity）
1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
存储（Storage）
Frozen in liquid nitrogen.
支原体检测（Mycoplasma Testing）
Negative
无菌检测（Sterility Testing）
Negative
使用说明（Instructions for Use）
See data sheet for detailed culturing and assay protocol.
Application
Inhibition of human Wnt surrogate protein-induced reporter activity by Wnt/β-catenin pathway Inhibitor.
This reporter cell was incubated with serial dilutions of inhibitors in the presence of human Wnt surrogate protein with a final concentration of 0.050 μg/mL. The EC50 of Wnt/β-catenin pathway Inhibitor (ICG001) was approximately 3.993 μM.
Protocol
Signaling Bioassay
Response to human Wnt surrogate protein (RLU).
This reporter cell was incubated with serial dilutions of human Wnt surrogate protein. The EC50 was approximately 0.02628 μg/mL.
Protocol
Response to human Wnt surrogate protein (FOLD).
This reporter cell was incubated with serial dilutions of human Wnt surrogate protein. The max induction fold was approximately 14.
Protocol
Passage Stability
Passage stability analysis by Signaling Bioassay.
The continuously growing TCF/LEF (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of human Wnt surrogate protein. Human Wnt surrogate protein stimulated response demonstrates passage stabilization (fold induction and EC50) across passage 7-21.
Protocol
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背景（Background）
T-cell factor/lymphoid enhancer factor (TCF/LEF) transcription factors are the major end point mediators of Wnt/Wingless signaling throughout metazoans. TCF/LEFs are multifunctional proteins that use their sequence-specific DNA-binding and context-dependent interactions to specify which genes will be regulated by Wnts.
License Disclosure
This cell line is provided for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make this cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit modification of this cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license. Modifications of this cell line, transfer to another facility, or commercial use of the cell lines may require a separate license and additional fees. AcroBiosystems does not warrant the suitability of this cell line for any particular use, and does not accept any liability in connection with the handling or use of this cell line.
