分子别名(Synonym)
Interleukin-10 receptor subunit alpha,IL-10 receptor subunit alpha,IL-10R subunit alpha,IL-10RA,CDw210a,Interleukin-10 receptor subunit 1,IL-10R subunit 1,IL-10R1,CD210,IL10RA,IL10R
表达区间及表达系统(Source)
Human IL-10 R alpha Protein, Fc Tag (ILR-H525a) is expressed from human 293 cells (HEK293). It contains AA His 22 - Asn 235 (Accession # Q13651).
Predicted N-terminus: His 22
Request for sequence
蛋白结构(Molecular Characterization)
This protein carries a human IgG1 Fc tag at the C-terminus
The protein has a calculated MW of 50.8 kDa. The protein migrates as 65-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
>95% as determined by SDS-PAGE.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳(SDS-PAGE)
Human IL-10 R alpha Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human IL-10 R alpha Protein, Fc Tag (Cat. No. ILR-H525a) is more than 85% and the molecular weight of this protein is around 125-150kDa verified by SEC-MALS.
Report
活性(Bioactivity)-ELISA
Immobilized Human IL-10, His Tag (Cat. No. IL0-H4248) at 5 μg/mL (100 μL/well) can bind Human IL-10 R alpha / CD210, Fc Tag (Cat. No. ILR-H525a) with a linear range of 0.2-2 ng/mL (QC tested).
Protocol
活性(Bioactivity)-BLI
Loaded Human IL-10 R alpha / CD210, Fc Tag (Cat. No. ILR-H525a) on Protein A Biosensor, can bind Human IL-10, His Tag (Cat. No. IL0-H4248) with an affinity constant of 1.5 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
背景(Background)
Interleukin-10 receptor subunit alpha (IL-10 R alpha ) is a cell surface receptor for the cytokine IL-10 that participates in IL-10 mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Upon binding to IL10, induces a conformational change in IL10RB, allowing IL10RB to bind IL10 as well.In turn, the heterotetrameric assembly complex, composed of two subunits of IL10RA and IL10RB, activates the kinases JAK1 and TYK2 that are constitutively associated with IL10RA and IL10RB respectively.These kinases then phosphorylate specific tyrosine residues in the intracellular domain in IL10RA leading to the recruitment and subsequent phosphorylation of STAT3.