膜杰作
Star Staining
分子别名（Synonym）
Integrin alpha V beta 6,ITGAV&ITGB6
表达区间及表达系统（Source）
Rat ITGAV&ITGB6 Heterodimer, His Tag&Tag Free (IT6-R52E6) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Pro 988 (ITGAM) & Gly 22 - Pro 708 (ITGB6) (Accession # NP_001385621.1 (ITGAM) & Q6AYF4 (ITGB6)).
Predicted N-terminus: Phe 31 & Gly 22
Request for sequence
蛋白结构（Molecular Characterization）
Rat ITGAV&ITGB6 Heterodimer, His Tag&Tag Free, has a calculated MW of 112.3 kDa (ITGAV) & 80.1 kDa (ITGB6). The protein migrates as 70-85 kDa and 125-145 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris,150 mM NaCl,pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Rat ITGAV&ITGB6 Heterodimer, His Tag&Tag Free on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Rat ITGAV&ITGB6 Heterodimer, His Tag&Tag Free (Cat. No. IT6-R52E6) is more than 85% and the molecular weight of this protein is around 242-272 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Human Fibronectin at 5 μg/mL (100 μL/well) can bind Rat ITGAV&ITGB6 Heterodimer, His Tag&Tag Free (Cat. No. IT6-R52E6) with a linear range of 0.02-0.625 μg/mL (QC tested).
Protocol
Immobilized Rat ITGAV&ITGB6 Heterodimer, His Tag&Tag Free (Cat. No. IT6-R52E6) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Latent TGF-Beta 1, His,Avitag (Cat. No. TG1-H82Qb) with a linear range of 0.3-10 ng/mL (Routinely tested).
Protocol
背景（Background）
Integrin alpha V beta 6 is a heterodimer of beta-6 associating with alpha-V. Integrin alpha-V beta-6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of integrin alpha-V beta-6 via clathrin-mediated endocytosis promotes carcinoma cell invasion. Also, Integrin alpha-V beta-6 acts as a receptor for coxsackievirus A9 and coxsackievirus B1 as well as herpes simplex virus-1/HHV-1. Furthermore, it binds the TGF-beta latency‑associated peptide (LAP) and activates TGF-beta 1 or TGF-beta 3 from large latent complexes. This activation requires interaction with LTBP-1 and fibronectin, and is enhanced by PAR-1.
