膜杰作
Star Staining
分子别名（Synonym）
CD45,PTPRC,L-CA,T200
表达区间及表达系统（Source）
This protein carries a human IgG2a Fc tag at the C-terminus. Human CD45, Llama IgG2b Fc Tag, low endotoxin (CD5-H5259) is expressed from human 293 cells (HEK293). It contains AA Gln 26 - Lys 577 (Accession # P08575-3).
Predicted N-terminus: Gln 26
Request for sequence
蛋白结构（Molecular Characterization）
This protein carries a llama IgG2b Fc tag at the C-terminus.
The protein has a calculated MW of 88.6 kDa. The protein migrates as 200-250 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 0.05 EU per μg by the LAL method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 200 mM Glycine, 25 mM Arginine, 150mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
电泳（SDS-PAGE）
Human CD45, Llama IgG2b Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
背景（Background）
CD45 is a receptor protein tyrosine phosphatase, also known as Ly-5 or leukocyte common antigen. CD45 mainly involves in the initiation of T cell receptor signaling by controlling the activation of the Src family protein-tyrosine kinases Lck and Fyn. CD45 deficiency causes in T- and B-lymphocyte dysfunction in the form of severe combined immune deficiency. It also takes a significant role in autoimmune diseases and cancer as well as in infectious diseases including fungal infections.
