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Rat Integrin alpha V beta 5 (ITGAV&ITGB5) Heterodimer Protein, His Tag&Tag Free

分子别名(Synonym)

Integrin alpha V beta 5,ITGAV&ITGB5

表达区间及表达系统(Source)

Rat ITGAV&ITGB5 Heterodimer, His Tag&Tag Free (IT5-R52E3) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Pro 988 (ITGAM) & Leu 25 - Asn 719 (ITGB5) (Accession # NP_001385621.1 (ITGAM) & NP_671480.2 (ITGB5)).

Predicted N-terminus: Phe 31 | Leu 25

Request for sequence

蛋白结构(Molecular Characterization)

Rat ITGAV&ITGB5 Heterodimer, His Tag&Tag Free, has a calculated MW of 112.3 kDa (ITGAV) & 81.6 kDa (ITGB3). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB3 contains no tag but a basic tail at the C-terminus.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

Integrin alpha V beta 5 SDS-PAGE

Rat ITGAV&ITGB5 Heterodimer, His Tag&Tag Free on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

Integrin alpha V beta 5 ELISA

Immobilized Human Vitronectin, Tag Free (Cat. No. VIN-H5119) at 5 μg/mL (100 μL/well) can bind Rat ITGAV&ITGB5 Heterodimer, His Tag&Tag Free (Cat. No. IT5-R52E3) with a linear range of 0.02-0.156 μg/mL (QC tested).

Protocol

Integrin alpha V beta 5 ELISA

Immobilized Biotinylated Human Vitronectin, Tag Free, primary amine labeling (Cat. No. VIN-H8113) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Rat ITGAV&ITGB5 Heterodimer, His Tag&Tag Free (Cat. No. IT5-R52E3) with a linear range of 0.078-0.625 μg/mL (Routinely tested).

Protocol

背景(Background)

Integrin alpha V beta 5 (ITGAV & ITGB5) is expressed on a wide variety of cell types including keratinocytes, fibroblasts, adhesive monocytes, embryonic stem cells, and select endothelium and epithelium. ITGAV & ITGB5 binds ligands containing an RGD motif, notably vitronectin. Growth factors that increase PKC activity, such as VEGF or TGF alpha, promote ITGAV & ITGB5-mediated angiogenesis while alpha V beta 3, which may be expressed in the same cell, responds to FGF-basic and TNF alpha. An inhibitor of both down regulates tumor angiogenesis. During lung inflammation, up regulation of ITGAV & ITGB5 on myofibroblasts or infiltrating lymphocytes may contribute to fibrosis by freeing TGF beta from latency.

 

 
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Integrin alpha V beta 5靶点信息
英文全称:Integrin alpha-V/beta-5
中文全称:整合素α-2/β-5复合体
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:0详情
最高研发阶段:临床前
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