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resDetect™ Human Interleukin-4 (IL-4) ELISA Kit (Residue Testing)

For research use only.

产品描述(Product Details)

Assay TypeSandwich-ELISA
AnalyteIL-4
Format96T
ReactivityHuman
Regulatory StatusRUO
Sensitivity< 0.391pg/mL
Standard Curve Range0.391 pg/mL-25 pg/mL
Assay Time2 hr 50 min
Suitable Sample TypeFor the quantitative determination of human IL-4 in Cell Culture Supernatants, Plasma, Serum.
Sample volume100 uL

组分(Materials Provided)

IDComponentsSize
CRS004-C01Pre-coated Anti-IL-4 Antibody Microplate1 plate
CRS004-C02Human IL-4 Standard20 μg
CRS004-C03Biotin-Anti-IL-4 Antibody50 μL
CRS004-C04Streptavidin-HRP50 μL
CRS004-C0510xWashing Buffer 50 mL
CRS004-C062xDilution Buffer50 mL
CRS004-C07Substrate Solution12 mL
CRS004-C08Stop Solution7 mL

背景(Background)

Human Interleukin-4 (IL-4) ELISA Kit (Residue Testing) is based on ELISA sandwich method and designed to detect and quantitative determine of GMP human IL-4 in samples from CAR-T product preparation processing. It contains GMP human IL-4 and a pair of antibodies against the recombinant factor, which are provided by ACROBiosystems. Results are obtained by four parameter logistic curve that were parallel to the standard curves obtained. The verification results indicate that this kit can be used for the quantitative determination of GMP human IL-4 (ACROBiosystems, cat#GMP-L04H26) concentrations. The specificity has been verified.

应用说明(Application)

Human Interleukin-4 (IL-4) ELISA Kit (Residue Testing) was developed for the detection and quantitative determination of GMP human IL-4 in samples from CAR-T product preparation processing.

It is for research use only.

重构方法(Reconstitution)

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

存储(Storage)

Unopened kit should be stored at 2°C -8°C upon receiving.

Find the expiration date on the outside packaging and do not use reagents past their expiration date.

The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

原理(Assay Principles)

This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of IL-4. The kit consists of Pre-coated Anti-IL-4 Antibody Microplate and Human IL-4 Standard and Biotin-Anti-IL-4 Antibody and Streptavidin-HRP and buffers.

Your experiment will include 6 simple steps:

a) Bring all reagents to room temperature(20℃-25℃) before use.

b) Add your sample to the plate and take the Human IL-4 as standard. The samples and standard are diluted by Dilution Buffer.

c) Wash the plate and add the Biotin-Anti-IL-4 Antibody diluted by Dilution Buffer to the plate.

d) Wash the plate and add the Streptavidin-HRP diluted by Dilution Buffer to the plate.

e) Wash the plate and add TMB.

f) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated by the absorbance at 450 nm minus the absorbance at 630 nm to remove background disturbance before statistical analysis. The OD Value reflects the amount of bound protein.

 

典型数据-Typical Data Please refer to Ds document for the assay protocol.

IL-4 TYPICAL DATA

For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.

 

验证(Validation)

稀释线性(Dilution Linearity)

To assess the linearity of the assay, samples spiked with high concentrations of human IL-4 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

IL-4 DILUTION LINEARITY

批内差异(Intra-Assay Statistics)

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision, Intra-Assay Precision CV<10%.

IL-4 INTRA-ASSAY STATISTICS

批间差异(Inter-Assay Statistics)

Three samples of known concentration were tested in three separate assays to assess inter-assay precision, Inter-Assay Precision CV<10%.

IL-4 INTER-ASSAY STATISTICS

回收率(Recovery)

Three parts of blank serum were added with different concentrations of human IL-4, and the serum without human IL-4 was used as background to calculate the recovery rate. The range of the recovery rate is 80.9-97.7%, and the average recovery is 86.3%.

IL-4 RECOVERY

 

背景(Background):IL-4

Interleukin-4, is a cytokine that induces differentiation of naive helper T cells (Th0 cells to Th2 cells). In the presence of IL-4 and IL-13, cytokines that are produced in a Th-2 type response, particularly during allergy and parasitic infections, macrophages become differentially activated, And this cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. Recently, researcher found that the cytokine IL-4 plays a key role in development of innate CD8+ T cells in the thymus of several gene-deficient mouse strains, including Itk, KLF2, CBP and Id3, without previous exposure to antigen.

 

前沿进展

 
 
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联系电话:
+86 400-682-2521(全国)
010-53681107(北京)
021-50850665(上海)
运输方式
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order.cn@acrobiosystems.com
技术支持邮箱:
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IL-4靶点信息
英文全称:Interleukin-4
中文全称:白细胞介素-4
种类:Homo sapiens
上市药物数量:1详情
临床药物数量:8详情
最高研发阶段:批准上市
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