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Biotinylated Human PSCA Protein, His,Avitag™ (MALS verified)

分子别名(Synonym)

PSCA,UNQ206,PRO232

表达区间及表达系统(Source)

Biotinylated Human PSCA, His,Avitag (PSA-H82E3) is expressed from human 293 cells (HEK293). It contains AA Leu 12 - Ser 86 (Accession # O43653-1).

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蛋白结构(Molecular Characterization)

PSCA Structure

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)

The protein has a calculated MW of 12.1 kDa. The protein migrates as 20-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Labeling)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;
  2. -70°C for 3 months under sterile conditions after reconstitution.
 

电泳(SDS-PAGE)

PSCA SDS-PAGE

Biotinylated Human PSCA, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS

PSCA SEC-MALS

The purity of Biotinylated Human PSCA, His,Avitag (Cat. No. PSA-H82E3) is more than 90% and the molecular weight of this protein is around 18-25 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

PSCA ELISA

Immobilized Biotinylated Human PSCA, His,Avitag (Cat. No. PSA-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-PSCA Antibody,Human IgG1 with a linear range of 0.1-2 ng/mL (QC tested).

Protocol

 

活性(Bioactivity)-FACS

PSCA FACS

2e5 of anti-PSCA CAR-293 cells were stained with 100 μL of 3 μg/mL of Biotinylated Human PSCA, His,Avitag (Cat. No. PSA-H82E3) and negative control protein respectively, washed and then followed by PE-SA antibody and analyzed with FACS (Routinely tested).

Protocol

 

背景(Background)

The Prostate stem cell antigen (PSCA) is a glycosylphosphatidylinositol (GPI)-anchored protein, plays an important role in tumorigenesis. The prostate stem cell antigen (PSCA) gene, which encodes a prostate-specific antigen (PSA), was identified as a gene involved in cell adhesion and proliferation. PSCA may be involved in the regulation of cell proliferation. Has a cell-proliferation inhibition activity in vitro. May act as a modulator of nicotinic acetylcholine receptors (nAChRs) activity. In vitro inhibits nicotine-induced signaling probably implicating alpha-3:beta-2- or alpha-7-containing nAChRs.

 

前沿进展

 
 
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PSCA靶点信息
英文全称:Prostate stem cell antigen
中文全称:前列腺干细胞抗原
种类:Homo sapiens
上市药物数量:0详情
临床药物数量:2详情
最高研发阶段:临床一期
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